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We have compared the relative abundance of estrogen receptor (ER)\u03b1, ER\u03b2, progesterone receptor (PR), and androgen receptor (AR) mRNA in four primary cultures of HOSE cells obtained from postmenopausal women to those found in late serous adenocarcinoma primary cell cultures and established ovarian cancer cell lines. We observed coexpression of ER\u03b1 and ER\u03b2 mRNA along with AR and PR transcripts in normal HOSE cells and disruption of ER\u03b1 mRNA expression as well as dramatic down-regulation of PR and AR transcript expression in most ovarian cancer cells. In contrast, levels of ER\u03b2 mRNA were unaffected by the malignant state. Additionally, a novel mutation involving a 32-bp deletion in exon 1 of ER\u03b1 transcripts was detected in the SKOV3 cell line. This mutation would explain why SKOV3 was reported to be ER-positive but estrogen-insensitive. Taken together, these findings suggest that estrogens, signaling via either or both ER subtypes, may play an indispensable role in regulating normal HOSE cell functions. Therefore, loss of ER\u03b1, PR, and AR mRNA expression in HOSE cells may be responsible for neoplastic transformation in this cell type. In contrast, the roles played by ER\u03b2 in normal and malignant HOSE cells remain elusive. Finally, the coexistence of mutated ER\u03b1 mRNA and normal ER\u03b2 transcripts in SKOV3 argues in favor of a dependency of ER\u03b2 action on functional ER\u03b1s.<\/jats:p>","DOI":"10.1073\/pnas.96.10.5722","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:40:52Z","timestamp":1027680052000},"page":"5722-5727","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":221,"title":["Expression of human estrogen receptor-\u03b1 and -\u03b2, progesterone receptor, and androgen receptor mRNA in normal and malignant ovarian epithelial cells"],"prefix":"10.1073","volume":"96","author":[{"given":"Kin-Mang","family":"Lau","sequence":"first","affiliation":[{"name":"Department of Biology, Tufts University, Medford, MA 02155; and Laboratory of Gynecological Oncology, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, MA 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