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In some cases, the mast cell TNF-\u03b1 response was triggered when these cells directly bound FimH on the surface of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . We have identified CD48, a glycosylphosphatidylinositol-anchored molecule, to be the complementary FimH-binding moiety in rodent mast cell membrane fractions. We showed that (\n                    <jats:italic>i<\/jats:italic>\n                    ) pretreatment of mast cell membranes with antibodies to CD48 or phospholipase C inhibited binding of FimH\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>E. coli<\/jats:italic>\n                    , (\n                    <jats:italic>ii<\/jats:italic>\n                    ) FimH\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>E. coli<\/jats:italic>\n                    but not a FimH\n                    <jats:sup>\u2212<\/jats:sup>\n                    derivative bound isolated CD48 in a mannose-inhibitable manner, (\n                    <jats:italic>iii<\/jats:italic>\n                    ) binding of FimH\n                    <jats:sup>+<\/jats:sup>\n                    bacteria to Chinese hamster ovary (CHO) cells was markedly increased when these cells were transfected with CD48 cDNA, and (\n                    <jats:italic>iv<\/jats:italic>\n                    ) antibodies to CD48 specifically blocked the mast cell TNF-\u03b1 response to FimH\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>E. coli.<\/jats:italic>\n                    Thus, CD48 is a functionally relevant microbial receptor on mast cells that plays a role in triggering inflammation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.96.14.8110","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:38:21Z","timestamp":1027679901000},"page":"8110-8115","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":200,"title":["The mast cell tumor necrosis factor \u03b1 response to FimH-expressing\n                    <i>Escherichia coli<\/i>\n                    is mediated by the glycosylphosphatidylinositol-anchored molecule CD48"],"prefix":"10.1073","volume":"96","author":[{"given":"Ravi","family":"Malaviya","sequence":"first","affiliation":[{"name":"Department of Pathology and Microbiology, Duke University Medical Center, Durham, NC 27710; and Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, Duke University Medical Center, Durham, NC 27710; and Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnan","family":"Thankavel","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, Duke University Medical Center, Durham, NC 27710; and Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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