{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:04:32Z","timestamp":1790251472134,"version":"4.1.0"},"reference-count":36,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1999,7,6]]},"abstract":"<jats:p>\n                    Vasodilator-stimulated phosphoprotein (VASP), a substrate of cAMP- and cGMP-dependent protein kinases, is associated with focal adhesions, cell\u2013cell contacts, microfilaments, and highly dynamic membrane regions. VASP, which is expressed in most cell types and in particularly high levels in human platelets, binds to profilin, zyxin, vinculin, F-actin, and the\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    surface protein ActA. VASP is a member of the enabled (Ena)\/VASP protein family and is thought to be involved in actin filament formation and integrin \u03b1\n                    <jats:sub>IIb<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    inhibition in human platelets. To gain further insight into the\n                    <jats:italic>in vivo<\/jats:italic>\n                    function of this protein, VASP-deficient mice were generated by homologous recombination. VASP\u2212\/\u2212 mice demonstrated hyperplasia of megakaryocytes in bone marrow and spleen but exhibited no other macroscopic or microscopic abnormalities. Activation of platelets with thrombin induced a more than 2-fold higher surface expression of P-selectin and fibrinogen binding in VASP-deficient platelets in comparison to wild type. These data support the concept that VASP is a negative modulator of platelet and integrin \u03b1\n                    <jats:sub>IIb<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    activation. Although the limited phenotypic differences between wild-type and VASP\u2212\/\u2212 mice suggested functional compensation of VASP by members of the Ena\/VASP family, alterations in the expression levels of mammalian enabled (Mena) and Ena-VASP-like (Evl) protein were not detected. VASP-deficient mice may provide an interesting model system for diseases in which enhanced platelet activation plays a major role.\n                  <\/jats:p>","DOI":"10.1073\/pnas.96.14.8120","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:38:21Z","timestamp":1027679901000},"page":"8120-8125","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":194,"title":["Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice"],"prefix":"10.1073","volume":"96","author":[{"given":"Wolfgang","family":"Hauser","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus-Peter","family":"Knobeloch","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Eigenthaler","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stepan","family":"Gambaryan","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veit","family":"Krenn","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Geiger","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Glazova","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Rohde","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Horak","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Walter","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Biochemie und Pathobiochemie und Pathologisches Institut der Universit\u00e4t W\u00fcrzburg, Josef-Schneider Strasse 2, 97080 W\u00fcrzburg, Germany; and Forschungsinstitut f\u00fcr Molekulare Pharmakologie, Abteilung Molekulare Genetik, Krahmerstrasse 6, 12207 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1999,7,6]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1987.tb13357.x"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb15079.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39737-6"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1992.tb16803.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)36652-8"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.32.20029"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00817-S"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.00021.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05264.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1093\/oso\/9780198599579.003.0054","volume-title":"Guidebook to the Cytoskeletal and Motor Proteins","author":"Reinhard M","year":"1999","unstructured":"M Reinhard, T Jarchau, K Reinhard, U Walter Guidebook to the Cytoskeletal and Motor Proteins, eds T Kreis, R Vale (Oxford Univ. Press, Oxford), pp. 168\u2013171 (1999)."},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb06971.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.5.521"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1996.0457"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81341-0"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.9.8.2157"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07146.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.17.7956"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.17.5433"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07117.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01295-1"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3180753"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi970065n"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.14.1.137"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81697-9"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.8.2645.2645_2645_2657"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90508-A"},{"key":"e_1_3_3_27_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"1989","unstructured":"J Sambrook, E F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, 2nd Ed., Plainview, NY, 1989).","edition":"2"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.7.1991"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118837"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112542"},{"key":"e_1_3_3_31_2","first-page":"283","volume":"46","author":"Meinecke M","year":"1994","unstructured":"M Meinecke, J Geiger, E Butt, M Sandberg, T Jahnsen, T Chakraborty, U Walter, T Jarchau, S M Lohmann Mol Pharmacol 46, 283\u2013290 (1994).","journal-title":"Mol Pharmacol"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(93)90006-B"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.1.37"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81092-2"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199212173272510"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199604253341710"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.96.14.8120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T23:57:07Z","timestamp":1704326227000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.96.14.8120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,7,6]]},"references-count":36,"journal-issue":{"issue":"14","published-print":{"date-parts":[[1999,7,6]]}},"alternative-id":["10.1073\/pnas.96.14.8120"],"URL":"https:\/\/doi.org\/10.1073\/pnas.96.14.8120","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,7,6]]},"assertion":[{"value":"1998-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-03-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-07-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}