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Microinjection of constitutively active V12CDC42Hs or a constitutively active WASp fragment consisting of the verprolin\/cofilin-like domains led to the disassemly of podosomes. Conversely, macrophages from patients expressing truncated forms of WASp completely lacked podosomes. These findings indicate that WASp controls podosome assembly and, in cooperation with CDC42Hs, podosome disassembly in primary human macrophages.<\/jats:p>","DOI":"10.1073\/pnas.96.17.9648","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"9648-9653","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":389,"title":["Wiskott-Aldrich syndrome protein regulates podosomes in primary human macrophages"],"prefix":"10.1073","volume":"96","author":[{"given":"Stefan","family":"Linder","sequence":"first","affiliation":[{"name":"Max von Pettenkofer-Institut f\u00fcr Medizinische Mikrobiologie and Institut f\u00fcr Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Pettenkoferstrasse 9a, Ludwig-Maximilians-Universit\u00e4t, 80336 Munich, Germany; National Cancer Institute, Metabolism Branch, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892; 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