{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:23:32Z","timestamp":1781105012432,"version":"3.54.1"},"reference-count":51,"publisher":"National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1999,1,19]]},"abstract":"<jats:p>\n                    The role of cytosolic ATP in exocytosis was investigated by using amperometric measurement of insulin exocytosis in pancreatic beta cells, which were stimulated with photolysis of caged Ca\n                    <jats:sup>2+<\/jats:sup>\n                    compounds. Insulin exocytosis occurred with two rates. We found that ATP hastened and augmented the exocytosis via selective enhancement of the exocytosis with the faster rate. A nonhydrolysable analog of ATP, adenosine 5\u2032-\n                    <jats:italic>O<\/jats:italic>\n                    -(3-thiotriphosphate), which blocks ATPase, was even more effective than ATP, indicating that the phosphorylation event occurred downstream of ATP-dependent vesicle transportation and priming. The action of ATP was eliminated by a competitive antagonist of cAMP, and by an inhibitor of adenylate cyclase. These data characterize an ATP sensing mechanism for the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -dependent exocytosis involving adenylate-cyclase, cAMP-dependent protein kinase, and, possibly, the fusion machinery itself. Thus, the fast exocytotic machinery requires both phosphorylation and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    for the final triggering and likely constitutes a distal ATP sensor for insulin exocytosis that acts in concert with ATP-sensitive K\n                    <jats:sup>+<\/jats:sup>\n                    channels.\n                  <\/jats:p>","DOI":"10.1073\/pnas.96.2.760","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:42:40Z","timestamp":1027680160000},"page":"760-765","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":132,"title":["Post-priming actions of ATP on Ca\n                    <sup>2+<\/sup>\n                    -dependent exocytosis in pancreatic beta cells"],"prefix":"10.1073","volume":"96","author":[{"given":"Noriko","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Physiology and Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; and Department of Physiology, MCP Hahnemann University, Philadelphia, Pennsylvania 19129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kadowaki","sequence":"additional","affiliation":[{"name":"Department of Physiology and Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; and Department of Physiology, MCP Hahnemann University, Philadelphia, Pennsylvania 19129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Yazaki","sequence":"additional","affiliation":[{"name":"Department of Physiology and Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; and Department of Physiology, MCP Hahnemann University, Philadelphia, Pennsylvania 19129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham C. 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