{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:12:12Z","timestamp":1791223932740,"version":"4.1.0"},"reference-count":37,"publisher":"National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1999,11,9]]},"abstract":"<jats:p>\n                    Axonal guidance is key to the formation of neuronal circuitry. Semaphorin 3A (Sema 3A; previously known as semaphorin III, semaphorin D, and collapsin-1), a secreted subtype of the semaphorin family, is an important axonal guidance molecule\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The molecular mechanisms of the repellent activity of semaphorins are, however, poorly understood. We have now found that the secreted semaphorins contain a short sequence of high homology to hanatoxin, a tarantula K\n                    <jats:sup>+<\/jats:sup>\n                    and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ion channel blocker. Point mutations in the hanatoxin-like sequence of Sema 3A reduce its capacity to repel embryonic dorsal root ganglion axons. Sema 3A growth cone collapse activity is inhibited by hanatoxin, general Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel blockers, a reduction in extracellular or intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , and a calmodulin inhibitor, but not by K\n                    <jats:sup>+<\/jats:sup>\n                    channel blockers. Our data support an important role for Ca\n                    <jats:sup>2+<\/jats:sup>\n                    in mediating the Sema 3A response and suggest that Sema 3A may produce its effects by causing the opening of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels.\n                  <\/jats:p>","DOI":"10.1073\/pnas.96.23.13501","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:39:15Z","timestamp":1027679955000},"page":"13501-13505","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":39,"title":["Semaphorin 3A growth cone collapse requires a sequence homologous to tarantula hanatoxin"],"prefix":"10.1073","volume":"96","author":[{"given":"Oded","family":"Behar","sequence":"first","affiliation":[{"name":"Neural Plasticity Research Group, Department of Anesthesia and Critical Care, Massachusetts General Hospital, and Cardiovascular Division, Children\u2019s Hospital, Harvard Medical School, Charlestown, MA 02129; and Department of Anatomy and Developmental Biology, University College London, London WCIE 6BT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Neural Plasticity Research Group, Department of Anesthesia and Critical Care, Massachusetts General Hospital, and Cardiovascular Division, Children\u2019s Hospital, Harvard Medical School, Charlestown, MA 02129; and Department of Anatomy and Developmental Biology, University College London, London WCIE 6BT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Badminton","sequence":"additional","affiliation":[{"name":"Neural Plasticity Research Group, Department of Anesthesia and Critical Care, Massachusetts General Hospital, and Cardiovascular Division, Children\u2019s Hospital, Harvard Medical School, Charlestown, MA 02129; and Department of Anatomy and Developmental Biology, University College London, London WCIE 6BT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clifford J.","family":"Woolf","sequence":"additional","affiliation":[{"name":"Neural Plasticity Research Group, Department of Anesthesia and Critical Care, Massachusetts General Hospital, and Cardiovascular Division, Children\u2019s Hospital, Harvard Medical School, Charlestown, MA 02129; and Department of Anatomy and Developmental Biology, University College London, London WCIE 6BT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1999,11,9]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050934"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80064-L"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90625-Z"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/383525a0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80368-2"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1999.0747"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19961118)375:3<378::AID-CNE3>3.0.CO;2-#"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903610209"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90333-X"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80534-6"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80535-8"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80392-X"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80626-1"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80672-8"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-06-01965.1999"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-16-06256.1997"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5382.1515"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90299-T"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90408-1"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/336775a0"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90184-1"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.15.8585"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.118.2.411"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90270-8"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-01-00184.1998"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80414-6"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5394.1705"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)60530-0"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-13-04973.1998"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)75272-7"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34513-1"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.74.3.375"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80697-2"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8418499"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/16927"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1298"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80369-4"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.96.23.13501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T15:33:16Z","timestamp":1649863996000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.96.23.13501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11,9]]},"references-count":37,"journal-issue":{"issue":"23","published-print":{"date-parts":[[1999,11,9]]}},"alternative-id":["10.1073\/pnas.96.23.13501"],"URL":"https:\/\/doi.org\/10.1073\/pnas.96.23.13501","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,11,9]]},"assertion":[{"value":"1999-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-11-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}