{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:39:11Z","timestamp":1781102351072,"version":"3.54.1"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"25","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1999,12,7]]},"abstract":"<jats:p>\n                    Recent experimental evidence suggests that reactive nitrogen oxide species can contribute significantly to postischemic myocardial injury. The aim of the present study was to evaluate the role of two reactive nitrogen oxide species, nitroxyl (NO\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) and nitric oxide (NO\n                    <jats:sup>\u22c5<\/jats:sup>\n                    ), in myocardial ischemia and reperfusion injury. Rabbits were subjected to 45 min of regional myocardial ischemia followed by 180 min of reperfusion. Vehicle (0.9% NaCl), 1 \u03bcmol\/kg\n                    <jats:italic>S-<\/jats:italic>\n                    nitrosoglutathione (GSNO) (an NO\n                    <jats:sup>\u22c5<\/jats:sup>\n                    donor), or 3 \u03bcmol\/kg Angeli\u2019s salt (AS) (a source of NO\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) were given i.v. 5 min before reperfusion. Treatment with GSNO markedly attenuated reperfusion injury, as evidenced by improved cardiac function, decreased plasma creatine kinase activity, reduced necrotic size, and decreased myocardial myeloperoxidase activity. In contrast, the administration of AS at a hemodynamically equieffective dose not only failed to attenuate but, rather, aggravated reperfusion injury, indicated by an increased left ventricular end diastolic pressure, myocardial creatine kinase release and necrotic size. Decomposed AS was without effect. Co-administration of AS with ferricyanide, a one-electron oxidant that converts NO\n                    <jats:sup>\u2212<\/jats:sup>\n                    to NO\n                    <jats:sup>\u22c5<\/jats:sup>\n                    , completely blocked the injurious effects of AS and exerted significant cardioprotective effects similar to those of GSNO. These results demonstrate that, although NO\n                    <jats:sup>\u22c5<\/jats:sup>\n                    is protective, NO\n                    <jats:sup>\u2212<\/jats:sup>\n                    increases the tissue damage that occurs during ischemia\/reperfusion and suggest that formation of nitroxyl may contribute to postischemic myocardial injury.\n                  <\/jats:p>","DOI":"10.1073\/pnas.96.25.14617","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:39:15Z","timestamp":1027679955000},"page":"14617-14622","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":143,"title":["Opposite effects of nitric oxide and nitroxyl on postischemic myocardial injury"],"prefix":"10.1073","volume":"96","author":[{"given":"Xin L.","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Surgery, Thomas Jefferson University, Philadelphia, PA 19107; Department of Pharmacology, University of California School of Medicine, Los Angeles, CA 90024; Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892; and The Wolfson Institute for Biomedical Research, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Surgery, Thomas Jefferson University, Philadelphia, PA 19107; Department of Pharmacology, University of California School of Medicine, Los Angeles, CA 90024; Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892; and The Wolfson Institute for Biomedical Research, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao-Lin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Surgery, Thomas Jefferson University, Philadelphia, PA 19107; Department of Pharmacology, University of California School of Medicine, Los Angeles, CA 90024; Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892; and The Wolfson Institute for Biomedical Research, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard L.","family":"Lopez","sequence":"additional","affiliation":[{"name":"Department of Surgery, Thomas Jefferson University, Philadelphia, PA 19107; Department of Pharmacology, University of California School of Medicine, Los Angeles, CA 90024; Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892; and The Wolfson Institute for Biomedical Research, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore A.","family":"Christopher","sequence":"additional","affiliation":[{"name":"Department of Surgery, Thomas Jefferson University, Philadelphia, PA 19107; Department of Pharmacology, University of California School of Medicine, Los Angeles, CA 90024; Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892; and The Wolfson Institute for Biomedical Research, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon M.","family":"Fukuto","sequence":"additional","affiliation":[{"name":"Department of Surgery, Thomas Jefferson University, Philadelphia, PA 19107; 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