{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:20:32Z","timestamp":1781104832084,"version":"3.54.1"},"reference-count":49,"publisher":"National Academy of Sciences","issue":"9","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1999,4,27]]},"abstract":"<jats:p>Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium-dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels\u2014cadmium, \u03c9-conotoxin MVIIC, \u03c9-agatoxin IVA, and \u03c9-agatoxin TK\u2014blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through \u03c9-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.<\/jats:p>","DOI":"10.1073\/pnas.96.9.5019","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:42:40Z","timestamp":1027680160000},"page":"5019-5024","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":40,"title":["Calcium influx is required for endocytotic membrane retrieval"],"prefix":"10.1073","volume":"96","author":[{"given":"Steven S.","family":"Vogel","sequence":"first","affiliation":[{"name":"Institute of Molecular Medicine and Genetics, Department of Medicine and Department of Pharmacology and Toxicology and Medical Research Service, Veterans Affairs Medical Center, Medical College of Georgia, 1120 15th Street CB 2803, Augusta, GA 30912-2630"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Smith","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine and Genetics, Department of Medicine and Department of Pharmacology and Toxicology and Medical Research Service, Veterans Affairs Medical Center, Medical College of Georgia, 1120 15th Street CB 2803, Augusta, GA 30912-2630"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Baibakov","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine and Genetics, Department of Medicine and Department of Pharmacology and Toxicology and Medical Research Service, Veterans Affairs Medical Center, Medical College of Georgia, 1120 15th Street CB 2803, Augusta, GA 30912-2630"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihide","family":"Ikebuchi","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine and Genetics, Department of Medicine and Department of Pharmacology and Toxicology and Medical Research Service, Veterans Affairs Medical Center, Medical College of Georgia, 1120 15th Street CB 2803, Augusta, GA 30912-2630"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nevin A.","family":"Lambert","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine and Genetics, Department of Medicine and Department of Pharmacology and Toxicology and Medical Research Service, Veterans Affairs Medical Center, Medical College of Georgia, 1120 15th Street CB 2803, Augusta, GA 30912-2630"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1999,4,27]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican1177-128"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.48.030186.001203"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/232341a0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/250155a0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.88.3.564"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.131.5.1183"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.87.1.297"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.5.667"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.18.8328"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80036-7"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.4.885"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-01-00081.1998"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.591bv.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(95)00126-Q"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(94)90353-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/370652a0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90081-2"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.11.5567"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1996.76.1.1"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.6.2343"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/380531a0"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90358-X"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(89)90210-8"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.5.1915"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(77)90084-7"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(78)90028-3"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90377-3"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.134.2.329"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1247\/csf.15.309"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.08.060179.002125"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1120"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90221-X"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/355827a0"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(94)90021-3"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-07-02259.1991"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90076-P"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1350109"},{"key":"e_1_3_3_38_2","volume-title":"Ionic Channels of Excitable Membranes","author":"Hille B","year":"1992","unstructured":"B Hille Ionic Channels of Excitable Membranes (Sinauer, Sunderland, MA, 1992)."},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74455-X"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(78)90103-3"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.8.3330"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/366156a0"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(95)80030-6"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(96)00195-1"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050441"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(96)80121-8"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(97)01083-7"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/261068a0"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90178-7"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.96.9.5019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T17:52:10Z","timestamp":1649872330000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.96.9.5019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,4,27]]},"references-count":49,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1999,4,27]]}},"alternative-id":["10.1073\/pnas.96.9.5019"],"URL":"https:\/\/doi.org\/10.1073\/pnas.96.9.5019","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,4,27]]},"assertion":[{"value":"1998-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-03-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-04-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}