{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:04:26Z","timestamp":1791176666782,"version":"4.1.0"},"reference-count":29,"publisher":"National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,1,18]]},"abstract":"<jats:p>\n                    The\n                    <jats:italic>copA<\/jats:italic>\n                    gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . The\n                    <jats:italic>copA<\/jats:italic>\n                    gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype could be rescued by complementation by a plasmid carrying\n                    <jats:italic>copA<\/jats:italic>\n                    from\n                    <jats:italic>E. coli<\/jats:italic>\n                    or\n                    <jats:italic>copB<\/jats:italic>\n                    from\n                    <jats:italic>Enterococcus hirae<\/jats:italic>\n                    . Expression of\n                    <jats:italic>copA<\/jats:italic>\n                    was induced by salts of copper or silver but not zinc or cobalt. Everted membrane vesicles from cells expressing\n                    <jats:italic>copA<\/jats:italic>\n                    exhibited ATP-coupled accumulation of copper, presumably as Cu(I). The results indicate that CopA is a Cu(I)-translocating efflux pump that is similar to the copper pumps related to Menkes and Wilson diseases and provides a useful prokaryotic model for these human diseases.\n                  <\/jats:p>","DOI":"10.1073\/pnas.97.2.652","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:38:21Z","timestamp":1027679901000},"page":"652-656","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":479,"title":["CopA: An\n                    <i>Escherichia coli<\/i>\n                    Cu(I)-translocating P-type ATPase"],"prefix":"10.1073","volume":"97","author":[{"given":"Christopher","family":"Rensing","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bharati","family":"Mitra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry P.","family":"Rosen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2000,1,18]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/129.7.1251"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5415.805"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006286"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1073"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.19.5891-5897.1999"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0193-7"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1293-327"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320110310"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)31455-8"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.20.9651"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08575.x"},{"key":"e_1_3_2_12_2","first-page":"123","volume":"643","author":"Melchers K","year":"1998","unstructured":"K Melchers, L Herrmann, F Mauch, D Bayle, D Heuermann, T Weitzenegger, A Schuhmacher, G Sachs, R Haas, G Bode, et al. 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