{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:04:23Z","timestamp":1791072263714,"version":"4.1.0"},"reference-count":15,"publisher":"National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,1,18]]},"abstract":"<jats:p>\n                    Genome projects are generating large numbers of potential new targets for drug discovery. One challenge is target validation, proving the usefulness of a specific target in an animal model. In this paper, we demonstrate a new approach to validation and assay development. We selected\n                    <jats:italic>in vitro<\/jats:italic>\n                    specific peptide binders to a potential pathogen target. By inducing the expression of a selected peptide in pathogen cells causing a lethal infection in mice, the animals were rescued. Thus, by combining\n                    <jats:italic>in vitro<\/jats:italic>\n                    selection methods for peptide binders with inducible expression in animals, the target's validity was rigorously tested and demonstrated. This approach to validation can be generalized and has the potential to become a valuable tool in the drug discovery process.\n                  <\/jats:p>","DOI":"10.1073\/pnas.97.2.783","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:41:48Z","timestamp":1027680108000},"page":"783-786","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":38,"title":["Drug target validation: Lethal infection blocked by inducible peptide"],"prefix":"10.1073","volume":"97","author":[{"given":"Jianshi","family":"Tao","sequence":"first","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Wendler","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene","family":"Connelly","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Lim","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"King","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongchuan","family":"Li","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared A.","family":"Silverman","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Schimmel","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis P.","family":"Tally","sequence":"additional","affiliation":[{"name":"Cubist Pharmaceuticals, Inc., 24 Emily Street, Cambridge, MA 02139; and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2000,1,18]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5331.1453"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7542800"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.20.6228-6237.1997"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-11-3097"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7618105"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5911966.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.15.8927"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90124-2"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/123.4.625"},{"key":"e_1_3_3_10_2","first-page":"498","volume-title":"The Enzymes","author":"S\u00f6ll D","year":"1974","unstructured":"D S\u00f6ll, P R Schimmel The Enzymes, ed P Boyer (Academic, 3rd. 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