{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:53:45Z","timestamp":1789214025348,"version":"build-2803163510"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"26","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,12,19]]},"abstract":"<jats:p>\n                    Serum amyloid P component (SAP) binds to\n                    <jats:italic>Streptococcus pyogenes<\/jats:italic>\n                    , and we show here that it also binds to\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    , including a lipopolysaccharide (LPS)-negative mutant, and to rough variants of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . Surprisingly, this binding had a powerful antiopsonic effect both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , reducing phagocytosis and killing of bacteria. Furthermore, SAP knockout mice survived lethal infection with\n                    <jats:italic>S. pyogenes<\/jats:italic>\n                    and rough\n                    <jats:italic>E. coli<\/jats:italic>\n                    J5, organisms to which SAP binds. The susceptibility of SAP\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice was fully restored by injection of isolated human SAP. However, SAP\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice were more susceptible than wild-type animals to lethal infection with\n                    <jats:italic>E. coli<\/jats:italic>\n                    O111:B4, a smooth strain to which SAP does not bind, suggesting that SAP also has some host defense function. Although SAP binds to LPS\n                    <jats:italic>in vitro<\/jats:italic>\n                    , SAP\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice were only marginally more susceptible to lethal LPS challenge, and injection of large amounts of human SAP into wild-type mice did not affect sensitivity to LPS, indicating that SAP is not a significant modulator of LPS toxicity\n                    <jats:italic>in vivo<\/jats:italic>\n                    . In contrast, the binding of SAP to pathogenic bacteria enabled them to evade neutrophil phagocytosis and display enhanced virulence. Abrogation of this molecular camouflage is thus potentially a novel therapeutic approach, and we show here that administration to wild-type mice of (\n                    <jats:italic>R<\/jats:italic>\n                    )-1-[6-(\n                    <jats:italic>R<\/jats:italic>\n                    )-2-carboxy-pyrrolidin-1-yl]-6-oxo-hexanoyl]pyrrolidine-2-carboxylic acid, a drug that inhibits SAP binding, significantly prolonged survival during lethal infection with\n                    <jats:italic>E. coli<\/jats:italic>\n                    J5.\n                  <\/jats:p>","DOI":"10.1073\/pnas.97.26.14584","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:44:19Z","timestamp":1027680259000},"page":"14584-14589","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":127,"title":["Role of serum amyloid P component in bacterial infection: Protection of the host or protection of the pathogen"],"prefix":"10.1073","volume":"97","author":[{"given":"Mahdad","family":"Noursadeghi","sequence":"first","affiliation":[{"name":"Centre for Amyloidosis and Acute Phase Proteins, Department of Medicine, Royal Free and University College Medical School, London NW3 2PF, United Kingdom; and Department of Infectious Diseases and Immunological Medicine Unit, Department of Medicine, Imperial College School of Medicine, Hammersmith Hospital, London W12 0NN, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C. 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