{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T14:39:55Z","timestamp":1784817595279,"version":"3.55.0"},"reference-count":44,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,3,28]]},"abstract":"<jats:p>\n                    The chlorophyll\n                    <jats:italic>a\/b<\/jats:italic>\n                    , chlorophyll\n                    <jats:italic>a\/c<\/jats:italic>\n                    , and chlorophyll\n                    <jats:italic>a\/a<\/jats:italic>\n                    light-harvesting proteins are part of an extended gene family that also includes the transiently expressed stress proteins, the Elips (early light-induced proteins). Four Elip homologue proteins, encoded by single-copy nuclear genes, have been identified in the\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    database. These proteins were divided into two groups according to the expression pattern under light-stress conditions and the predicted secondary structure. Group one included two members of the Elip family with three predicted transmembrane helices and a gene expression strictly related to light stress. Group two included two proteins, the Seps (stress-enhanced proteins), which possessed two predicted transmembrane segments. The transcripts of Sep1 and Sep2 were present under low light conditions, but their level increased 4- to 10-fold during illumination of plants with high-intensity light. Preliminary data indicated that the induced transcripts were translated\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Other physiological stress conditions, such as cold, heat, desiccation, salt, wounding, or oxidative stress, did not significantly influence the expression of\n                    <jats:italic>Sep<\/jats:italic>\n                    genes.\n                    <jats:italic>In vitro<\/jats:italic>\n                    import of radioactively labeled precursors of Seps into isolated chloroplasts confirmed the thylakoid membrane localization of these proteins. Considering the predicted protein structure and homology to other pigment-antenna proteins, the two-helix Seps might represent an evolutionary missing link between the one- and three-helix antenna proteins present in pro- and eukaryota.\n                  <\/jats:p>","DOI":"10.1073\/pnas.97.7.3741","type":"journal-article","created":{"date-parts":[[2012,7,25]],"date-time":"2012-07-25T13:37:36Z","timestamp":1343223456000},"page":"3741-3746","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":168,"title":["Light stress-regulated two-helix proteins in\n                    <i>Arabidopsis thaliana<\/i>\n                    related to the chlorophyll\n                    <i>a\/b<\/i>\n                    -binding gene family"],"prefix":"10.1073","volume":"97","author":[{"given":"Mounia","family":"Heddad","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-10691 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Adamska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-10691 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2000,3,21]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1038\/367566a0","volume":"367","author":"Wolfe R G","year":"1994","unstructured":"R G Wolfe, F X Cunningham, D Durnford, B R Green, E Gantt Nature (London) 367, 566\u2013568 (1994).","journal-title":"Nature (London)"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/PL00006445","volume":"48","author":"Durnford D G","year":"1999","unstructured":"D G Durnford, J A Deane, S Tan, G I McFadden, E Gantt, B R Green J Mol Evol 48, 59\u201368 (1999).","journal-title":"J Mol Evol"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/S1360-1385(99)01419-3","volume":"4","author":"Jansson S","year":"1999","unstructured":"S Jansson Trends Plant Sci 4, 236\u2013240 (1999).","journal-title":"Trends Plant Sci"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"11133","DOI":"10.1021\/bi00035a019","volume":"34","author":"Funk C","year":"1995","unstructured":"C Funk, W Schr\u00f6der, A Napiwotzki, S Tjus, G Renger, B Andersson Biochemistry 34, 11133\u201311141 (1995).","journal-title":"Biochemistry"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0014-5793(92)81463-V","volume":"314","author":"Kim S","year":"1992","unstructured":"S Kim, P Sandusky, N R Bowlby, R Aebersold, B R Green, S Vlahakis, C F Yokum, E Pichersky FEBS Lett 314, 67\u201371 (1992).","journal-title":"FEBS Lett"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0014-5793(92)81462-U","volume":"314","author":"Wedel N","year":"1992","unstructured":"N Wedel, R Klein, U Ljungberg, B Andersson, R G Herrmann FEBS Lett 314, 61\u201366 (1992).","journal-title":"FEBS Lett"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0968-0004(91)90072-4","volume":"16","author":"Green B R","year":"1991","unstructured":"B R Green, E Pichersky, K Kloppstech Trends Biochem Sci 16, 181\u2013186 (1991).","journal-title":"Trends Biochem Sci"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1073\/pnas.89.7.2610","volume":"89","author":"Adamska I","year":"1992","unstructured":"I Adamska, I Ohad, K Kloppstech Proc Natl Acad Sci USA 89, 2610\u20132613 (1992).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1073\/pnas.92.2.636","volume":"92","author":"Miroshnichenko-Dolganov N A","year":"1995","unstructured":"N A Miroshnichenko-Dolganov, D Bhaya, A R Grossmann Proc Natl Acad Sci USA 92, 636\u2013640 (1995).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"9397","DOI":"10.1021\/bi990545+","volume":"38","author":"Funk C","year":"1999","unstructured":"C Funk, W Vermaas Biochemistry 38, 9397\u20139404 (1999).","journal-title":"Biochemistry"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/BF00018304","volume":"44","author":"Green B R","year":"1995","unstructured":"B R Green, W K\u00fchlbrandt Photosynth Res 44, 139\u2013148 (1995).","journal-title":"Photosynth Res"},{"key":"e_1_3_4_12_2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/j.1432-1033.1984.tb07900.x","volume":"138","author":"Meyer G","year":"1984","unstructured":"G Meyer, K Kloppstech Eur J Biochem 138, 201\u2013207 (1984).","journal-title":"Eur J Biochem"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/BF00027318","volume":"13","author":"Grimm B","year":"1989","unstructured":"B Grimm, E Kruse, K Kloppstech Plant Mol Biol 13, 583\u2013593 (1989).","journal-title":"Plant Mol Biol"},{"key":"e_1_3_4_14_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/BF00020553","volume":"16","author":"Adamska I","year":"1991","unstructured":"I Adamska, K Kloppstech Plant Mol Biol 16, 209\u2013223 (1991).","journal-title":"Plant Mol Biol"},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1023\/A:1005920730947","volume":"54","author":"Lindahl M","year":"1997","unstructured":"M Lindahl, C Funk, J Webster, S Bingsmark, I Adamska, B Andersson Photosynth Res 54, 227\u2013236 (1997).","journal-title":"Photosynth Res"},{"key":"e_1_3_4_16_2","doi-asserted-by":"crossref","first-page":"5438","DOI":"10.1016\/S0021-9258(18)53340-8","volume":"268","author":"Adamska I","year":"1993","unstructured":"I Adamska, K Kloppstech, I Ohad J Biol Chem 268, 5438\u20135444 (1993).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_17_2","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1104\/pp.115.3.1287","volume":"115","author":"Moscovici-Kadouri S","year":"1997","unstructured":"S Moscovici-Kadouri, D A Chamovitz Plant Physiol 115, 1287\u20131290 (1997).","journal-title":"Plant Physiol"},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/978-94-011-3953-3_92","volume-title":"Photosynthesis: Mechanisms and Effects","author":"Heddad M","year":"1998","unstructured":"M Heddad, I Adamska Photosynthesis: Mechanisms and Effects, ed G Garab (Kluwer, Dordrecht, The Netherlands), pp. 389\u2013392 (1998)."},{"key":"e_1_3_4_19_2","first-page":"217","volume":"24","author":"Altschul S F","year":"1997","unstructured":"S F Altschul, T L Madden, A A Sch\u00e4ffer, J Zhang, Z Zhang, W Miller, D J Lipman Nucleic Acids Res 24, 217\u2013221 (1997).","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_4_20_2","doi-asserted-by":"crossref","first-page":"12098","DOI":"10.1073\/pnas.93.22.12098","volume":"93","author":"Morgenstern B","year":"1996","unstructured":"B Morgenstern, A Dress, T Werner Proc Natl Acad Sci USA 93, 12098\u201312103 (1996).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1007\/BF00329648","volume":"209","author":"Kolanus W","year":"1987","unstructured":"W Kolanus, C Scharnhorst, U K\u00fchne, F Herzfeld Mol Gen Genet 209, 234\u2013239 (1987).","journal-title":"Mol Gen Genet"},{"key":"e_1_3_4_22_2","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1271\/bbb.61.2143","volume":"61","author":"Yamagata H","year":"1996","unstructured":"H Yamagata, C Bowler Biosci Biotechnol Biochem 61, 2143\u20132144 (1996).","journal-title":"Biosci Biotechnol Biochem"},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1007\/BF00019469","volume":"31","author":"Ouvrard O","year":"1996","unstructured":"O Ouvrard, F Cellier, K Ferrare, D Tousch, T Lamaze, J M Dupuis, F Casse-Delbart Plant Mol Biol 31, 819\u2013829 (1996).","journal-title":"Plant Mol Biol"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"2771","DOI":"10.1002\/j.1460-2075.1992.tb05344.x","volume":"11","author":"Bartels D","year":"1992","unstructured":"D Bartels, C Hanke, K Schneider, D Michel, F Salamini EMBO J 11, 2771\u20132778 (1992).","journal-title":"EMBO J"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/BF02669187","volume":"13","author":"Reith M E","year":"1995","unstructured":"M E Reith, J Munholland Plant Mol Biol Rep 13, 333\u2013335 (1995).","journal-title":"Plant Mol Biol Rep"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1007\/BF00020019","volume":"18","author":"Kessler U","year":"1992","unstructured":"U Kessler, U Maid, K Zetsche Plant Mol Biol 18, 777\u2013780 (1992).","journal-title":"Plant Mol Biol"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"13698","DOI":"10.1016\/S0021-9258(18)92755-9","volume":"266","author":"Lers A","year":"1991","unstructured":"A Lers, H Levy, A Zamir J Biol Chem 266, 13698\u201313705 (1991).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/S0888-7543(05)80111-9","volume":"14","author":"Nakai K","year":"1992","unstructured":"K Nakai, M Kanehisa Genomics 14, 897\u2013911 (1992).","journal-title":"Genomics"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1110\/ps.8.5.978","volume":"8","author":"Emanuelsson O","year":"1999","unstructured":"O Emanuelsson, H Nielsen, G von Heijne Protein Sci 8, 978\u2013984 (1999).","journal-title":"Protein Sci"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/protein\/9.2.133","volume":"9","author":"Frishman D","year":"1996","unstructured":"D Frishman, P Argos Protein Eng. 9, 133\u2013142 (1996).","journal-title":"Protein Eng."},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/S0076-6879(96)66034-0","volume":"266","author":"Garnier J","year":"1996","unstructured":"J Garnier, J F Gibrat, B Robson Methods Enzymol 266, 540\u2013553 (1996).","journal-title":"Methods Enzymol"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1093\/protein\/10.6.673","volume":"10","author":"Cserzo L","year":"1997","unstructured":"L Cserzo, L Wallin, G Simon, G von Heijne, A Elofsson Protein Eng 10, 673\u2013676 (1997).","journal-title":"Protein Eng"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1093\/bioinformatics\/14.4.378","volume":"4","author":"Hirokawa S","year":"1998","unstructured":"S Hirokawa, S Boon-Chieng, S Mitaku BioInformatics 4, 378\u2013379 (1998).","journal-title":"BioInformatics"},{"key":"e_1_3_4_34_2","first-page":"175","volume-title":"Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology","author":"Sonnhammer E L L","year":"1998","unstructured":"E L L Sonnhammer, G von Heijne, A Krogh Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology, eds J Glasgow, T Littlejohn, F Major, R Lathrop, D Sankoff, C Sensen (AAAI, Menlo Park, CA), pp. 175\u2013182 (1998)."},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-2836(82)90515-0","volume":"157","author":"Kyle J","year":"1982","unstructured":"J Kyle, R F Doolittle J Mol Biol 157, 105\u2013132 (1982).","journal-title":"J Mol Biol"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/protein\/7.2.195","volume":"7","author":"Attwood T K","year":"1994","unstructured":"T K Attwood, J B C Findlay Protein Eng 7, 195\u2013203 (1994).","journal-title":"Protein Eng"},{"key":"e_1_3_4_37_2","first-page":"1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"1989","unstructured":"J Sambrook, E F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 1\u20135.78, \u20135.79. (1989)."},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"Laemmli U K","year":"1970","unstructured":"U K Laemmli Nature (London) 227, 680\u2013685 (1970).","journal-title":"Nature (London)"},{"key":"e_1_3_4_39_2","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1104\/pp.106.4.1241","volume":"106","author":"Newman T","year":"1994","unstructured":"T Newman, F J Bruijn, P Green, K Keegstra, H Kende, L McIntosh, J Ohlrogge, N Raikhel, S Somerville, M Thomashow, et al. Plant Physiol 106, 1241\u20131255 (1994).","journal-title":"Plant Physiol"},{"key":"e_1_3_4_40_2","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1038\/367614a0","volume":"367","author":"K\u00fchlbrandt W","year":"1994","unstructured":"W K\u00fchlbrandt, D N Wang, Y Fujiyoshi Nature (London) 367, 614\u2013621 (1994).","journal-title":"Nature (London)"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/BF00029382","volume":"39","author":"Green B R","year":"1994","unstructured":"B R Green, E Pichersky Photosynth Res 39, 149\u2013162 (1994).","journal-title":"Photosynth Res"},{"key":"e_1_3_4_42_2","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1111\/j.1432-1033.1993.tb17980.x","volume":"214","author":"P\u00f6tter E","year":"1993","unstructured":"E P\u00f6tter, K Kloppstech Eur J Biochem 214, 779\u2013786 (1993).","journal-title":"Eur J Biochem"},{"key":"e_1_3_4_43_2","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1046\/j.1432-1327.1999.00178.x","volume":"260","author":"Adamska I","year":"1999","unstructured":"I Adamska, M Roobol-B\u00f3za, M Lindahl, B Andersson Eur J Biochem 260, 453\u2013460 (1999).","journal-title":"Eur J Biochem"},{"key":"e_1_3_4_44_2","doi-asserted-by":"crossref","first-page":"15244","DOI":"10.1073\/pnas.93.26.15244","volume":"93","author":"La Roche J","year":"1996","unstructured":"J La Roche, G W M van der Staay, F Partensky, A Ducret, R Aebersold, R Li, S S Golden, R G Hiller, P M Wrench, A W D Larkum, et al. Proc Natl Acad Sci USA 93, 15244\u201315248 (1996).","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.97.7.3741","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T01:04:29Z","timestamp":1654563869000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.97.7.3741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3,21]]},"references-count":44,"aliases":["10.1073\/pnas.050391397","10.1073\/pnas.050391397"],"journal-issue":{"issue":"7","published-print":{"date-parts":[[2000,3,28]]}},"alternative-id":["10.1073\/pnas.97.7.3741"],"URL":"https:\/\/doi.org\/10.1073\/pnas.97.7.3741","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3,21]]},"assertion":[{"value":"1999-09-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-03-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}