{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T17:02:17Z","timestamp":1781110937337,"version":"3.54.1"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"1","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,1,2]]},"abstract":"<jats:p>\n                    Activation of phospholipase C in nonexcitable cells causes the release of calcium (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ) from intracellular stores and activation of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx by means of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release-activated channels (I\n                    <jats:sub>CRAC<\/jats:sub>\n                    ) in the plasma membrane. The molecular identity and the mechanism of I\n                    <jats:sub>CRAC<\/jats:sub>\n                    channel activation are poorly understood. Using the patch\u2013clamp technique, here we describe the plasma membrane Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels in human carcinoma A431 cells, which can be activated by extracellular UTP, by depletion of intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    stores after exposure to the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -pump inhibitor thapsigargin, or by loading the cells with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    chelator 1,2-bis(2-aminophenoxy)ethane-\n                    <jats:italic>N,N,N<\/jats:italic>\n                    \u2032\n                    <jats:italic>,N<\/jats:italic>\n                    \u2032-tetraacetate. The observed channels display the same conductance and gating properties as previously described I\n                    <jats:sub>min<\/jats:sub>\n                    channels, but have significantly lower conductance for monovalent cations than the I\n                    <jats:sub>CRAC<\/jats:sub>\n                    channels. Thus, we concluded that the depletion-activated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    current in A431 cells is supported by I\n                    <jats:sub>CRAC<\/jats:sub>\n                    -like (I\n                    <jats:sub>CRACL<\/jats:sub>\n                    ) channels, identical to I\n                    <jats:sub>min<\/jats:sub>\n                    . We further demonstrated synergism in activation of I\n                    <jats:sub>CRACL<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels by extracellular UTP and intracellular inositol (1,4,5)-triphosphate (IP\n                    <jats:sub>3<\/jats:sub>\n                    ), apparently because of reduction in phosphatidylinositol 4,5-bisphosphate (PIP\n                    <jats:sub>2<\/jats:sub>\n                    ) levels in the patch. Prolonged exposure of patches to thapsigargin renders I\n                    <jats:sub>CRACL<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels unresponsive to IP\n                    <jats:sub>3<\/jats:sub>\n                    but still available to activation by the combined action of IP\n                    <jats:sub>3<\/jats:sub>\n                    and anti-PIP\n                    <jats:sub>2<\/jats:sub>\n                    antibody. Based on these data, we concluded that phospholipase C-mediated and store-operated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx pathways in A431 cells converge on the same I\n                    <jats:sub>CRACL<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel, which can be modulated by PIP\n                    <jats:sub>2<\/jats:sub>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.98.1.148","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:45:09Z","timestamp":1027680309000},"page":"148-153","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":27,"title":["Activation of calcium entry in human carcinoma A431 cells by store depletion and phospholipase C- dependent mechanisms converge on I\n                    <sub>CRAC<\/sub>\n                    -like calcium channels"],"prefix":"10.1073","volume":"98","author":[{"given":"Elena","family":"Kaznacheyeva","sequence":"first","affiliation":[{"name":"Institute of Cytology RAS, 4 Tikhoretsky Avenue, St. Petersburg 194064, Russia; and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Zubov","sequence":"additional","affiliation":[{"name":"Institute of Cytology RAS, 4 Tikhoretsky Avenue, St. Petersburg 194064, Russia; and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Gusev","sequence":"additional","affiliation":[{"name":"Institute of Cytology RAS, 4 Tikhoretsky Avenue, St. Petersburg 194064, Russia; and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya","family":"Bezprozvanny","sequence":"additional","affiliation":[{"name":"Institute of Cytology RAS, 4 Tikhoretsky Avenue, St. Petersburg 194064, Russia; and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina N.","family":"Mozhayeva","sequence":"additional","affiliation":[{"name":"Institute of Cytology RAS, 4 Tikhoretsky Avenue, St. Petersburg 194064, Russia; and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2001,1,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3120001"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1997.77.4.901"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199901)21:1<38::AID-BIES5>3.0.CO;2-S"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/355353a0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"5226","DOI":"10.4049\/jimmunol.152.11.5226","volume":"152","author":"Premack B A","year":"1994","unstructured":"B A Premack, T V McDonald, P Gardner J Immunol 152, 5226\u20135240 (1994).","journal-title":"J Immunol"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.13.6295"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5403.836"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80132-4"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.26.15195"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80853-B"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00366-9"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050784"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/326301a0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/355356a0"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.3.C733"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.25983"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.7.4561"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/24890"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80344-5"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.2060"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.14955"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5458.1647"},{"key":"e_1_3_3_23_2","unstructured":"Estacion M. 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