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Despite its\n importance, the enzyme catalyzing the key step in vitamin A formation\n resisted molecular analyses until recently, when the successful cloning\n of a cDNA encoding an enzyme with \u03b2,\u03b2-carotene-15,15\u2032-dioxygenase\n activity from\n                    <jats:italic>Drosophila<\/jats:italic>\n                    was reported. To prove its\n identity with the key enzyme for vitamin A formation\n                    <jats:italic>in\n vivo<\/jats:italic>\n                    , we analyzed the blind\n                    <jats:italic>Drosophila<\/jats:italic>\n                    mutant\n                    <jats:italic>ninaB<\/jats:italic>\n                    . In two independent\n                    <jats:italic>ninaB<\/jats:italic>\n                    alleles,\n we found mutations in the gene encoding the\n \u03b2,\u03b2-carotene-15,15\u2032-dioxygenase. These mutations lead to a defect\n in vitamin A formation and are responsible for blindness of these\n flies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.98.3.1130","type":"journal-article","created":{"date-parts":[[2012,7,26]],"date-time":"2012-07-26T19:06:14Z","timestamp":1343329574000},"page":"1130-1135","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":14,"title":["Analysis of the blind\n                    <i>Drosophila<\/i>\n                    mutant\n                    <i>ninaB<\/i>\n                    identifies the gene encoding the key enzyme for vitamin A formation\n                    <i>in vivo<\/i>"],"prefix":"10.1073","volume":"98","author":[{"given":"Johannes","family":"von Lintig","sequence":"first","affiliation":[{"name":"Albert\u2013Ludwig University of Freiburg, Institute for Biology I,\r Neurobiology and Animal Physiology, Hauptstrasse 1, D-79104 Freiburg,\r 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