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To better understand the enzymology of hemoglobin degradation, it is necessary to determine which of these genes are expressed when hemoglobin degradation is occurring, which encode active enzymes, and which gene products are found in the food vacuole where catabolism takes place. Our genome-wide analysis reveals that PM I, II, and IV and histo-aspartic protease encode hemoglobin-degrading food vacuole proteases. Despite having a histidine in place of one of the catalytic aspartic acids conserved in other aspartic proteases, histo-aspartic protease is an active hydrolase.<\/jats:p>","DOI":"10.1073\/pnas.022630099","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:36:44Z","timestamp":1027694204000},"page":"990-995","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":397,"title":["Four plasmepsins are active in the<i>Plasmodium falciparum<\/i>food vacuole, including a protease with an active-site histidine"],"prefix":"10.1073","volume":"99","author":[{"given":"Ritu","family":"Banerjee","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8230, St. Louis, MO 63110"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8230, St. Louis, MO 63110"}]},{"given":"Wandy","family":"Beatty","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8230, St. Louis, MO 63110"}]},{"given":"Lorraine","family":"Pelosof","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8230, St. Louis, MO 63110"}]},{"given":"Michael","family":"Klemba","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8230, St. Louis, MO 63110"}]},{"given":"Daniel E.","family":"Goldberg","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8230, St. Louis, MO 63110"}]}],"member":"341","published-online":{"date-parts":[[2002,1,8]]},"reference":[{"key":"e_1_3_3_1_2","volume-title":"Malaria: Parasite Biology, Pathogenesis and Protection","author":"Trigg P I","year":"1998","unstructured":"P I Trigg, A V Kondrachine Malaria: Parasite Biology, Pathogenesis and Protection (Am. 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