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This method can be used in a simple, wide-field (nonscanning) microscope, uses only a single, inexpensive laser, and requires no unusual photophysical properties of the fluorophore. The practical resolving power is determined by the signal-to-noise ratio, which in turn is limited by photobleaching. Experimental results show that a 2D point resolution of &lt;50 nm is possible on sufficiently bright and photostable samples.<\/jats:p>","DOI":"10.1073\/pnas.0406877102","type":"journal-article","created":{"date-parts":[[2005,9,3]],"date-time":"2005-09-03T00:34:42Z","timestamp":1125707682000},"page":"13081-13086","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1900,"title":["Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution"],"prefix":"10.1073","volume":"102","author":[{"given":"Mats G. 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