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Twenty-four adenine auxotrophs were identified that exhibited supersensitivity to<jats:italic>A. tumefaciens<\/jats:italic>-mediated transformation when deprived of adenine. We extended these results to plants by showing that purine synthesis inhibitors cause supersensitivity to<jats:italic>A. tumefaciens<\/jats:italic>transformation in three plant species. The magnitude of this effect is large and does not depend on prior genetic manipulations of host cells. These data indicate the utility of yeast as a model for the transformation process and identify purine biosynthesis as a key determinant of transformation efficiency. These findings should increase the utility of<jats:italic>A. tumefaciens<\/jats:italic>in genetic engineering.<\/jats:p>","DOI":"10.1073\/pnas.1132022100","type":"journal-article","created":{"date-parts":[[2003,5,27]],"date-time":"2003-05-27T17:10:32Z","timestamp":1054055432000},"page":"6634-6639","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":27,"title":["Purine synthesis and increased<i>Agrobacterium tumefaciens<\/i>transformation of yeast and plants"],"prefix":"10.1073","volume":"100","author":[{"given":"Radclyffe L.","family":"Roberts","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle, WA 98195-7242"}]},{"given":"Matthew","family":"Metz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle, WA 98195-7242"}]},{"given":"Dave E.","family":"Monks","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle, WA 98195-7242"}]},{"given":"Matthew Lockwood","family":"Mullaney","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle, WA 98195-7242"}]},{"given":"Tyler","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle, WA 98195-7242"}]},{"given":"Eugene W.","family":"Nester","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle, WA 98195-7242"}]}],"member":"341","published-online":{"date-parts":[[2003,5,9]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.4.1613"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0998-839"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/315525"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004380050948"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S1016-8478(23)17116-0","volume":"12","year":"2001","unstructured":"Rho, H. 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