{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:52:59Z","timestamp":1779346379759,"version":"3.51.4"},"reference-count":40,"publisher":"Proceedings of the National Academy of Sciences","issue":"21","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,10,14]]},"abstract":"<jats:p>\n            Pathogenic bacteria secrete proteins that promote invasion of host tissues and resistance to immune responses. However, secretion mechanisms that contribute to the enormous morbidity and mortality of Gram-positive bacteria are largely undefined. An auxiliary protein secretion system (SecA2) has recently emerged in\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            and eight other Gram-positive pathogens. Here, a proteomics approach identified seventeen SecA2-dependent secreted and surface proteins of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            , the two most abundant of which [the p60 and\n            <jats:italic>N<\/jats:italic>\n            -acetylmuramidase (NamA) autolysins] hydrolyze bacterial peptidoglycan (PGN) and contribute to host colonization. SecA2-deficient (\u0394SecA2) bacteria were rapidly cleared after systemic infection of murine hosts, and in cultured cells showed reduced cell-cell spread. p60 or NamA deficiencies (\u0394p60 and \u0394NamA) caused intermediate reductions in bacterial virulence\n            <jats:italic>in vivo<\/jats:italic>\n            , yet showed no defect for infection of cultured cells. Restoration of virulence in \u0394p60 bacteria required full-length p60 with an intact catalytic domain, suggesting that PGN hydrolysis by p60 is crucial for\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            virulence. Coordinated PGN hydrolysis by p60 and NamA activities is predicted to generate a muramyl glycopeptide, glucosaminylmuramyl dipeptide (GMDP), which is known to modify host inflammatory responses. Thus, SecA2-dependent secretion may promote release of muramyl peptides that subvert host pattern recognition.\n          <\/jats:p>","DOI":"10.1073\/pnas.2133653100","type":"journal-article","created":{"date-parts":[[2003,10,14]],"date-time":"2003-10-14T19:05:46Z","timestamp":1066158346000},"page":"12432-12437","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":231,"title":["SecA2-dependent secretion of autolytic enzymes promotes\n            <i>Listeria monocytogenes<\/i>\n            pathogenesis"],"prefix":"10.1073","volume":"100","author":[{"given":"Laurel L.","family":"Lenz","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology and School of Public Health, University of California, 401 Barker Hall, Berkeley, CA 94720-3202"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sina","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology and School of Public Health, University of California, 401 Barker Hall, Berkeley, CA 94720-3202"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aimee","family":"Geissler","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology and School of Public Health, University of California, 401 Barker Hall, Berkeley, CA 94720-3202"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Portnoy","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology and School of Public Health, University of California, 401 Barker Hall, Berkeley, CA 94720-3202"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2003,10,3]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1104"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5458.1655"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.23.12638"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.2396"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg006"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(00)00111-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00198-2"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.896801"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.24.6979-6990.2001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02949.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03072.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03438.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01211.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.15.4177-4186.2002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2001.02208.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5953-5957.2001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200201081"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063447"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.11.3491-3501.1993"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200205009"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.12.3608-3613.1996"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.5.1555-1561.1999"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.19.11409"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi015798t"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.187-207.2001"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.10.3108-3116.2001"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3473-3484.2003"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-145-1-57"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.7.3.277"},{"key":"e_1_3_2_30_2","first-page":"1857","volume":"352","year":"1998","unstructured":"Bhushan, M., McElhone, K. & Griffiths, C. 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