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The changes we see are similar to those observed after treatment with LY294002, an inhibitor of phosphatidylinositol 3-OH kinase, fully consistent with the model that PTEN antagonizes phosphatidylinositol 3-OH kinase. Both treatments result in suppressed expression of the transforming growth factor (TGF)-\u03b2 gene and the genes of the cholesterol biosynthesis pathway. Our results illustrate the power of using a fully inducible expression system in conjunction with cDNA microarray analysis for exploring gene function.<\/jats:p>","DOI":"10.1073\/pnas.221450598","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:31:44Z","timestamp":1027693904000},"page":"13043-13048","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor"],"prefix":"10.1073","volume":"98","author":[{"given":"Javor","family":"Stolarov","sequence":"first","affiliation":[{"name":"Cold Spring Harbor Laboratory, 1 Bungtown Road, P.O. 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