{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:05:10Z","timestamp":1761764710310},"reference-count":0,"publisher":"Proceedings of the National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1972,7]]},"abstract":"<jats:p>\n            The almost complete purification of the previously reported DNA polymerase of chicken embryo is described; in the final isolation step the enrichment of the enzyme, which comprises about 0.01% of the protein extractable from 10-day-old embryos, is more than 9000-fold. The enzyme is a basic protein (pI = 9.15), of molecular weight about 27,000. It exhibits no nuclease activity, requires Mn\n            <jats:sup>2+<\/jats:sup>\n            ion for activity, and may contain Zn. It is very sensitive to oxidation.\n          <\/jats:p>","DOI":"10.1073\/pnas.69.7.1781","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T06:37:02Z","timestamp":1149057422000},"page":"1781-1785","update-policy":"http:\/\/dx.doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":33,"title":["DNA Polymerase of Chicken Embryo: Purification and Properties"],"prefix":"10.1073","volume":"69","author":[{"given":"Jannis G.","family":"Stavrianopoulos","sequence":"first","affiliation":[{"name":"Cell Chemistry Laboratory, Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, N.Y. 10032"}]},{"given":"John D.","family":"Karkas","sequence":"additional","affiliation":[{"name":"Cell Chemistry Laboratory, Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, N.Y. 10032"}]},{"given":"Erwin","family":"Chargaff","sequence":"additional","affiliation":[{"name":"Cell Chemistry Laboratory, Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, N.Y. 10032"}]}],"member":"341","published-online":{"date-parts":[[1972,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.69.7.1781","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:21:22Z","timestamp":1649859682000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.69.7.1781"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1972,7]]}},"alternative-id":["10.1073\/pnas.69.7.1781"],"URL":"https:\/\/doi.org\/10.1073\/pnas.69.7.1781","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,7]]},"assertion":[{"value":"1972-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}