{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T14:51:55Z","timestamp":1770475915641,"version":"3.49.0"},"reference-count":0,"publisher":"Proceedings of the National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1979,11]]},"abstract":"<jats:p>A transducing phage has been isolated with codes for the sigma subunit of Escherichia coli RNA polymerase. Transducing phage were selected from E. coli shotgun collections of HindIII or Sac I fragments cloned into Charon 25, a new bacteriophage lambda vector that is capble of forming lyosogens at high temperature. Transduction of an E. coli strain carrying a temperature-sensitive mutation in the sigma gene was used for the selection. The positions of restriction sites for Sac I, HindIII, Xho I, Bgl II, and Kpn I in the cloned bacterial DNA segments were determined. Phage containing the HindIII fragment complement both primase (dnaG) and sigma (rpoD) whereas those containing the Sac I fragment complement only sigma. Results of analyses of the proteins made both in vivo after infection of UV-irradiated cells and in vitro in a coupled transcription\/translation system suggest that a Sac I site separates the promoter for sigma from the sigma structural gene. The direction of transcription of sigma was determined to be clockwise with respect to the E. coli genetic map.<\/jats:p>","DOI":"10.1073\/pnas.76.11.5789","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:52:03Z","timestamp":1149061923000},"page":"5789-5793","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":17,"title":["Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase."],"prefix":"10.1073","volume":"76","author":[{"given":"C A","family":"Gross","sequence":"first","affiliation":[]},{"given":"F R","family":"Blattner","sequence":"additional","affiliation":[]},{"given":"W E","family":"Taylor","sequence":"additional","affiliation":[]},{"given":"P A","family":"Lowe","sequence":"additional","affiliation":[]},{"given":"R R","family":"Burgess","sequence":"additional","affiliation":[]}],"member":"341","published-online":{"date-parts":[[1979,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.76.11.5789","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:51:50Z","timestamp":1649861510000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.76.11.5789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1979,11]]}},"alternative-id":["10.1073\/pnas.76.11.5789"],"URL":"https:\/\/doi.org\/10.1073\/pnas.76.11.5789","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,11]]},"assertion":[{"value":"1979-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}