{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:49:38Z","timestamp":1781102978648,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1979,4]]},"abstract":"<jats:p>\n                    Purified human peripheral blood T lymphocytes were fractionated by density gradient electrophoresis on the basis of their surface charge. The high mobility cell fractions were found to be enriched in T cells having receptors for IgM (T\u03bc cells) with only minor proportions of T cells having receptors for IgG (T\u03b3 cells). In contrast, the low mobility cell fractions were enriched in T\u03b3 cells with very low numbers of contaminating T\u03bc cells. Both populations were of higher mobility than human peripheral blood B lymphocytes. High-affinity sheep erythrocyte resette-forming cells (E-RFC) were relatively enriched in the high and intermediate mobility fractions and appear to include the T\u03bc cells and the T cells without receptors for IgG or IgM (T\u03d5). The low affinity E-RFC were found only in the lower mobility fractions that included the T\u03b3 cell population. A direct correlation was observed between the number of T\u03b3 lymphocytes and the low affinity E-RFC in all the fractions. The separated cell fractions were treated\n                    <jats:italic>in vitro<\/jats:italic>\n                    with different concentrations of concanavalin A (Con A) and examined for the numbers of T\u03bc and T\u03b3 cells. Low concentrations of Con A (2.5 \u03bcg\/ml) significantly increased the number of T\u03bc cells, whereas high concentrations of Con A (20 \u03bcg\/ml and 40 \u03bcg\/ml) markedly reduced the number of T\u03bc cells and increased the number of T\u03b3 cells. Furthermore, all fractions (both T\u03bc and T\u03b3 cell enriched) responded by proliferation to Con A, whereas only the high and intermediate mobility fractions (enriched in T\u03bc cells) responded to phytohemagglutinin. Fractions enriched in T\u03b3 cells responded very poorly to phytohemagglutinin. This method provides another technique for separating human T\u03bc- and T\u03b3-enriched lymphocyte subpopulations and does not modulate the Fc receptors of the cells, in contrast to the rosetting techniques currently in use for the separation of these lymphocytes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.76.4.1972","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T03:58:12Z","timestamp":1149047892000},"page":"1972-1976","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":23,"title":["Separation of human T lymphocyte subpopulations (T\u03bc, T\u03b3) by density gradient electrophoresis"],"prefix":"10.1073","volume":"76","author":[{"given":"Chris D.","family":"Platsoucas","sequence":"first","affiliation":[{"name":"Memorial Sloan-Kettering Cancer Center, New York, New York 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Good","sequence":"additional","affiliation":[{"name":"Memorial Sloan-Kettering Cancer Center, New York, New York 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhir","family":"Gupta","sequence":"additional","affiliation":[{"name":"Memorial Sloan-Kettering Cancer Center, New York, New York 10021"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1979,4]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.76.4.1972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:28:07Z","timestamp":1649849287000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.76.4.1972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1979,4]]}},"alternative-id":["10.1073\/pnas.76.4.1972"],"URL":"https:\/\/doi.org\/10.1073\/pnas.76.4.1972","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,4]]},"assertion":[{"value":"1979-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}