{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:39:25Z","timestamp":1781102365583,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1980,6]]},"abstract":"<jats:p>\n                    Slow reacting substance(s) of anaphylaxis (SRS-A) was isolated from both human (lung) and rat sources and compared with three synthetic SRS-As of known structure\u2014leukotrienes (LTs) C-1, C-2, and D. Reversed-phase liquid chromatography was used both as a final purification step and a means of comparison of biologically derived and synthetic substances. Two major peaks of SRS-A activity of both rat and human origin corresponded chromatographically with LTC-1 and LTD, respectively, and had equivalent specific activities on the guinea pig ileum. With guinea pig ileum, the specific activities (units\/pmol) for synthetic leukotrienes and anaphylactic peaks were (mean \u00b1 SEM): synthetic LTC-1, 1.93 \u00b1 0.13; SRS-A\n                    <jats:sup>rat<\/jats:sup>\n                    peak I, 1.69 \u00b1 0.43; synthetic LTD, 6.10 \u00b1 1.15; SRS-A\n                    <jats:sup>rat<\/jats:sup>\n                    peak II, 7.14 \u00b1 0.51; and SRS-A\n                    <jats:sup>hu<\/jats:sup>\n                    peak II, 1.90. Both synthetic LTC-1 and LTD and their SRS-A natural counterparts had a preferential contractile activity on guinea pig peripheral airway compared to central airways and were at least 200 times more active than histamine on peripheral airways on a molar basis. Leukotriene D is the major SRS-A of human lung and accounts for almost all of the biological activity. It likely is formed from leukotriene C-1\n                    <jats:italic>in vivo<\/jats:italic>\n                    by an enzymic process of the well-known \u03b3-glutamyltransferase type.\n                  <\/jats:p>","DOI":"10.1073\/pnas.77.6.3710","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T04:21:20Z","timestamp":1149049280000},"page":"3710-3714","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":270,"title":["Slow reacting substances of anaphylaxis: Identification of leukotrienes C-1 and D from human and rat sources"],"prefix":"10.1073","volume":"77","author":[{"given":"Robert A.","family":"Lewis","sequence":"first","affiliation":[{"name":"Department of Medicine, Harvard Medical School and Robert B. Brigham Division of the Affiliated Hospitals Center, Inc., Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Frank","family":"Austen","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harvard Medical School and Robert B. Brigham Division of the Affiliated Hospitals Center, Inc., Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Drazen","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harvard Medical School and Robert B. Brigham Division of the Affiliated Hospitals Center, Inc., Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Marfat","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. J.","family":"Corey","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1980,6]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.77.6.3710","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:19:44Z","timestamp":1649848784000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.77.6.3710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1980,6]]}},"alternative-id":["10.1073\/pnas.77.6.3710"],"URL":"https:\/\/doi.org\/10.1073\/pnas.77.6.3710","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,6]]},"assertion":[{"value":"1980-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}