{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:57:01Z","timestamp":1781107021414,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1983,3]]},"abstract":"<jats:p>\n                    r(ts107)202 was isolated in HeLa cells as a temperature-independent revertant of H5ts107, an adenovirus mutant that maps in the structural gene of the viral DNA-binding protein. r(ts107)202 is a host-range temperature-conditional mutant: it is temperature independent for growth in HeLa cells but temperature sensitive for growth in 293 cells, a type 5 adenovirus-transformed human cell line. Marker rescue experiments using H5ts107 DNA and restriction enzyme fragments from r(ts107)202 DNA demonstrated that the mutations causing the r(ts107)202 phenotype were localized in\n                    <jats:italic>Hin<\/jats:italic>\n                    dIII fragment A containing the entire DNA-binding protein gene. To obtain a fine structure map of the r(ts107)202 mutations, overlap recombination between\n                    <jats:italic>Eco<\/jats:italic>\n                    RI fragment A (0-75.9 map units) from either Ad5wt or r(ts107)202 and\n                    <jats:italic>Bam<\/jats:italic>\n                    HI fragment B (59.5-100 map units) from either Ad5wt or r(ts107)202 was performed. Segregation of the H5ts107 primary-site mutation away from the accompanying reversion mutation could be demonstrated in 5 of 200 plaques when r(ts107)202\n                    <jats:italic>Eco<\/jats:italic>\n                    RI fragment A was crossed with the Ad5wt\n                    <jats:italic>Bam<\/jats:italic>\n                    HI fragment. In the reciprocal cross, none of 200 plaques contained the H5ts107 mutant. These results permitted a determination of the order of the primary-site mutation (H5ts107) and secondary-site mutation in r(ts107)202, and the frequency of recombination predicted a distance of 50-340 base pairs between these two mutations. This experimental result agrees with the nucleotide sequence of the r(ts107)202 mutant which shows that 182 base pairs separate the primary-site and secondary-site mutations in r(ts107)202.\n                  <\/jats:p>","DOI":"10.1073\/pnas.80.6.1674","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T05:19:53Z","timestamp":1149052793000},"page":"1674-1677","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":5,"title":["Detection, rescue, and mapping of mutations in the adenovirus DNA binding protein gene"],"prefix":"10.1073","volume":"80","author":[{"given":"J. C.","family":"Nicolas","sequence":"first","affiliation":[{"name":"Hopital Trousseau, 24 rue Arnold Netter, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. S. H.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Physicians and Surgeons, Columbia University, New York, New York 10027"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Suarez","sequence":"additional","affiliation":[{"name":"Pasteur Institute, 25 rue due Dr. Roux, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Girard","sequence":"additional","affiliation":[{"name":"Pasteur Institute, 25 rue due Dr. Roux, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J.","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, State University of New York, Stony Brook, New York 11794"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1983,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.80.6.1674","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:46:30Z","timestamp":1649850390000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.80.6.1674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,3]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1983,3]]}},"alternative-id":["10.1073\/pnas.80.6.1674"],"URL":"https:\/\/doi.org\/10.1073\/pnas.80.6.1674","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,3]]},"assertion":[{"value":"1983-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}