{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:51:13Z","timestamp":1781110273411,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1991,7]]},"abstract":"<jats:p>We have isolated a cDNA encoding a third type of protein-tyrosine-phosphatase. We screened human megakaryoblastic cell line (MEG-01) an umbilical vein endothelial cell cDNA libraries to obtain a 3.7-kilobase cDNA designated PTPase MEG. Northern blot analysis of MEG-01 RNA detected a 3.7-kilobase transcript, suggesting that a full-length cDNA has been identified. PTPase MEG cDNA contains an open reading frame of 926 amino acids. The cDNA has a G+C-rich 5' untranslated region of 771 nucleotides that has the potential to form stable stem-loop structures and has two upstream ATG codons. The predicted protein (Mr = 105,910) has no apparent membrane-spanning region and contains a single protein-tyrosine-phosphatase domain (amino acids 659-909) that is 35-40% identical to previously described tyrosine-phosphatase domains. The recombinant phosphatase domain possesses protein-tyrosine-phosphatase activity when expressed in Escherichia coli. The amino-terminal region (amino acids 31-367) is 45% identical to the amino terminus of human erythrocyte protein 4.1, a cytoskeletal protein. The identification of a protein-tyrosine-phosphatase that is related to cytoskeletal proteins implies that cell signaling activities reside not only in transmembrane receptors but in cytoskeletal elements as well.<\/jats:p>","DOI":"10.1073\/pnas.88.13.5867","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:40:52Z","timestamp":1149061252000},"page":"5867-5871","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":120,"title":["Identification, cloning, and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to cytoskeletal protein 4.1."],"prefix":"10.1073","volume":"88","author":[{"given":"M X","family":"Gu","sequence":"first","affiliation":[{"name":"Washington University School of Medicine, Division of Hematology-Oncology, St. Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J D","family":"York","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine, Division of Hematology-Oncology, St. Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Warshawsky","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine, Division of Hematology-Oncology, St. Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P W","family":"Majerus","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine, Division of Hematology-Oncology, St. Louis, MO 63110."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1991,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.88.13.5867","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:18:42Z","timestamp":1649855922000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.88.13.5867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,7]]},"references-count":0,"journal-issue":{"issue":"13","published-print":{"date-parts":[[1991,7]]}},"alternative-id":["10.1073\/pnas.88.13.5867"],"URL":"https:\/\/doi.org\/10.1073\/pnas.88.13.5867","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,7]]},"assertion":[{"value":"1991-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}