{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:33:22Z","timestamp":1781105602034,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"21","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,11]]},"abstract":"<jats:p>The respiratory burst oxidase of phagocytes and B lymphocytes is a complicated enzyme that catalyzes the one-electron reduction of oxygen by NADPH. It is responsible for the O2- production that occurs when these cells are exposed to phorbol 12-myristate 13-acetate or other appropriate stimuli. The activity of this enzyme is greatly decreased or absent in patients with chronic granulomatous disease, an inherited disorder characterized by a severe defect in host defense against bacteria and fungi. In every chronic granulomatous disease patient studied to date, an abnormality has been found in a gene encoding one of four components of the respiratory burst oxidase: the membrane protein p22phox or gp91phox, or the cytosolic protein p47phox or p67phox. We report here that O2- production was partly restored to phorbol 12-myristate 13-acetate-stimulated Epstein-Barr virus-transformed B lymphocytes from a patient with p47phox-deficient chronic granulomatous disease by transfection with an expression plasmid containing a p47phox cDNA inserted in the sense direction. No detectable O2- was produced by untransfected p47phox-deficient lymphocytes or by p47phox-deficient lymphocytes transfected with an antisense plasmid. The finding that O2- can be produced by p47phox-deficient B lymphocytes after the transfer of a p47phox cDNA into the deficient cells suggests that this system could be useful for studying the function of mutant p47phox proteins in whole cells.<\/jats:p>","DOI":"10.1073\/pnas.89.21.10174","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:11:21Z","timestamp":1149063081000},"page":"10174-10177","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":28,"title":["O2- production by B lymphocytes lacking the respiratory burst oxidase subunit p47phox after transfection with an expression vector containing a p47phox cDNA."],"prefix":"10.1073","volume":"89","author":[{"given":"S J","family":"Chanock","sequence":"first","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L R","family":"Faust","sequence":"additional","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Barrett","sequence":"additional","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Bizal","sequence":"additional","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F E","family":"Maly","sequence":"additional","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P E","family":"Newburger","sequence":"additional","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J M","family":"Ruedi","sequence":"additional","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B M","family":"Babior","sequence":"additional","affiliation":[{"name":"Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.21.10174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:49:22Z","timestamp":1649857762000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.21.10174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11]]},"references-count":0,"journal-issue":{"issue":"21","published-print":{"date-parts":[[1992,11]]}},"alternative-id":["10.1073\/pnas.89.21.10174"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.21.10174","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11]]},"assertion":[{"value":"1992-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}