{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:05:45Z","timestamp":1781103945589,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,12,15]]},"abstract":"<jats:p>In budding yeast, two transcription factors, Swi4 and Swi6, control the expression of important cell cycle regulatory proteins (the G1 cyclins, Cln1 and Cln2, and the cyclin-like Hcs26) as well as the HO gene, whose product initiates mating-type switching. Both Swi4 and Swi6 are components of a protein complex that forms at a repeated sequence element, SCB (SWI4, -6-dependent cell cycle box), found in the upstream regulatory sequences of target genes. We show, by using proteins synthesized in vitro, a direct association between Swi4 and Swi6. The cdc10-Swi6 or ankyrin motifs present in both Swi4 and Swi6 are dispensable for their association, which is mediated instead by a region near the C terminus of each protein. Furthermore, we show that interaction with Swi6 is not necessary for specific recognition of the SCB sequence by the Swi4 protein; we propose that Swi4 is responsible for binding to the SCB sequence while Swi6, through its association with Swi4, regulates activity of the complex.<\/jats:p>","DOI":"10.1073\/pnas.89.24.11852","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:14:57Z","timestamp":1149063297000},"page":"11852-11856","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":64,"title":["Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro."],"prefix":"10.1073","volume":"89","author":[{"given":"B J","family":"Andrews","sequence":"first","affiliation":[{"name":"Department of Molecular and Medical Genetics, University of Toronto, ON, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L A","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Genetics, University of Toronto, ON, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,12,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.24.11852","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:31:57Z","timestamp":1649856717000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.24.11852"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,12,15]]},"references-count":0,"journal-issue":{"issue":"24","published-print":{"date-parts":[[1992,12,15]]}},"alternative-id":["10.1073\/pnas.89.24.11852"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.24.11852","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,12,15]]},"assertion":[{"value":"1992-12-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}