{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:33:25Z","timestamp":1781105605595,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,3,15]]},"abstract":"<jats:p>Cloning and sequencing of chicken C-terminal Src kinase (CSK), a tyrosine kinase that phosphorylates the regulatory C-terminal tyrosine residue present on cytoplasmic tyrosine kinases of the Src family, demonstrated a high degree of interspecies conservation as well as src homology 2 and 3 domains N-terminal to the kinase domain. The lack of autophosphorylation sites distinguishes CSK from other tyrosine kinases. CSK is unique and does not belong to a gene family, suggesting that it may phosphorylate other members of the Src family of tyrosine kinases in addition to c-Src. Since complex formation between c-Src and CSK seemed a likely regulatory step in the control of c-Src kinase activity, such an association was investigated by immunoprecipitation and Western blotting as well as intracellular localization studies. Although some portions of CSK were found in a membrane fraction, no complex formation between CSK and c-Src was observed, suggesting that the src homology 2 domain of CSK does not play a role in the direct interaction of c-Src.<\/jats:p>","DOI":"10.1073\/pnas.89.6.2190","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:18:24Z","timestamp":1149063504000},"page":"2190-2194","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":68,"title":["Molecular cloning and expression of chicken C-terminal Src kinase: lack of stable association with c-Src protein."],"prefix":"10.1073","volume":"89","author":[{"given":"H","family":"Sabe","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021-6399."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Knudsen","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021-6399."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Okada","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021-6399."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Nada","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021-6399."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021-6399."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Hanafusa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021-6399."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,3,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.6.2190","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:41:07Z","timestamp":1649857267000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.6.2190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,3,15]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992,3,15]]}},"alternative-id":["10.1073\/pnas.89.6.2190"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.6.2190","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,3,15]]},"assertion":[{"value":"1992-03-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}