{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:42:20Z","timestamp":1781106140762,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1992,4,15]]},"abstract":"<jats:p>Overexpression of the p53 protein, resulting from gene mutations that increase protein stability, has been detected in greater than 25% of primary human breast cancers. In addition, approximately 10% of breast cancer patients have circulating antibodies to the p53 protein. In this study, the anti-p53 humoral response is correlated with the presence and type of mutant p53 protein expressed in the tumor. In a series of 60 breast cancer patients, 0 of 30 tumors with normal, low-level p53 expression induced anti-p53 antibodies, whereas 7 (23%) of 30 tumors with p53 overexpression elicited a specific anti-p53 antibody response. These 7 patients had anti-p53 antibodies that recognized wild-type p53 and a variety of mutant p53 proteins. A comparison of p53 mutations revealed that antibody-negative tumors had mutations exclusively in exons 7 and 8, whereas antibody-positive tumors had mutations primarily in exons 5 and 6. Moreover, all antibody-eliciting tumors contained complexes between p53 and a 70-kDa heat shock protein, whereas none of the antibody-negative tumors contained this complex. This study implicates a 70-kDa heat shock protein in the antigenic presentation of p53.<\/jats:p>","DOI":"10.1073\/pnas.89.8.3439","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T08:19:52Z","timestamp":1149063592000},"page":"3439-3442","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":171,"title":["Immune response to p53 is dependent upon p53\/HSP70 complexes in breast cancers."],"prefix":"10.1073","volume":"89","author":[{"given":"A M","family":"Davidoff","sequence":"first","affiliation":[{"name":"Department of Surgery, Duke University Medical Center, Durham, NC 27710."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J D","family":"Iglehart","sequence":"additional","affiliation":[{"name":"Department of Surgery, Duke University Medical Center, Durham, NC 27710."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J R","family":"Marks","sequence":"additional","affiliation":[{"name":"Department of Surgery, Duke University Medical Center, Durham, NC 27710."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1992,4,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.89.8.3439","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:41:02Z","timestamp":1649857262000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.89.8.3439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4,15]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1992,4,15]]}},"alternative-id":["10.1073\/pnas.89.8.3439"],"URL":"https:\/\/doi.org\/10.1073\/pnas.89.8.3439","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4,15]]},"assertion":[{"value":"1992-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}