{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T12:43:02Z","timestamp":1770813782312,"version":"3.50.1"},"reference-count":0,"publisher":"Proceedings of the National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:p>The T-cell receptor (TCR) beta chain is instrumental in the progression of thymocyte differentiation from the CD4-CD8- to the CD4+CD8+ stage. This differentiation step may involve cell surface expression of novel CD3-TCR complexes. To facilitate biochemical characterization of these complexes, we established cell lines from thymic lymphomas originating from mice carrying a mutation in the p53 gene on the one hand and a mutation in TCR-alpha, TCR-beta, or the recombination activating gene 1 (RAG-1) on the other hand. The cell lines were CD4+CD8+ and appeared to be monoclonal. A cell line derived from a RAG-1 x p53 double mutant thymic lymphoma expressed low levels of CD3-epsilon, -gamma, and -delta on the surface. TCR-alpha x p53 double mutant cell lines were found to express complexes consisting of TCR-beta chains associated with CD3-epsilon, -gamma, and -delta chains and CD3-zeta zeta dimers. These lines will be useful tools to study the molecular structure and signal transducing properties of partial CD3-TCR complexes expressed on the surface of immature thymocytes.<\/jats:p>","DOI":"10.1073\/pnas.92.16.7420","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T13:17:14Z","timestamp":1149081434000},"page":"7420-7424","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":35,"title":["Characterization of immature thymocyte lines derived from T-cell receptor or recombination activating gene 1 and p53 double mutant mice."],"prefix":"10.1073","volume":"92","author":[{"given":"P","family":"Mombaerts","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA."}]},{"given":"C","family":"Terhorst","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA."}]},{"given":"T","family":"Jacks","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA."}]},{"given":"S","family":"Tonegawa","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA."}]},{"given":"J","family":"Sancho","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA."}]}],"member":"341","published-online":{"date-parts":[[1995,8]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.16.7420","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T18:08:08Z","timestamp":1649873288000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.16.7420"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8]]},"references-count":0,"journal-issue":{"issue":"16","published-print":{"date-parts":[[1995,8]]}},"alternative-id":["10.1073\/pnas.92.16.7420"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.16.7420","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,8]]},"assertion":[{"value":"1995-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}