{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:21:50Z","timestamp":1762100510585},"reference-count":0,"publisher":"Proceedings of the National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:p>Chronic myelogenous leukemia evolves in two clinically distinct stages: a chronic and a blast crisis phase. The molecular changes associated with chronic phase to blast crisis transition are largely unknown. We have identified a cDNA clone, DR-nm23, differentially expressed in a blast-crisis cDNA library, which has approximately 70% sequence similarity to the putative metastatic suppressor genes, nm23-H1 and nm23-H2. The deduced amino acid sequence similarity to the proteins encoded by these two latter genes is approximately 65% and includes domains and amino acid residues (the leucine zipper-like and the RGD domain, a serine and a histidine residue in the NH2- and in the COOH-terminal portion of the protein, respectively) postulated to be important for nm23 function. DR-nm23 mRNA is preferentially expressed at early stages of myeloid differentiation of highly purified CD34+ cells. Its constitutive expression in the myeloid precursor 32Dc13 cell line, which is growth-factor dependent for both proliferation and differentiation, results in inhibition of granulocytic differentiation induced by granulocyte colony-stimulating factor and causes apoptotic cell death. These results are consistent with a role for DR-nm23 in normal hematopoiesis and raise the possibility that its overexpression contributes to differentiation arrest, a feature of blastic transformation in chronic myelogenous leukemia.<\/jats:p>","DOI":"10.1073\/pnas.92.16.7435","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T13:17:14Z","timestamp":1149081434000},"page":"7435-7439","update-policy":"http:\/\/dx.doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":121,"title":["Overexpression of DR-nm23, a protein encoded by a member of the nm23 gene family, inhibits granulocyte differentiation and induces apoptosis in 32Dc13 myeloid cells."],"prefix":"10.1073","volume":"92","author":[{"given":"D","family":"Venturelli","sequence":"first","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]},{"given":"R","family":"Martinez","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]},{"given":"P","family":"Melotti","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]},{"given":"I","family":"Casella","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]},{"given":"C","family":"Peschle","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]},{"given":"C","family":"Cucco","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]},{"given":"G","family":"Spampinato","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]},{"given":"Z","family":"Darzynkiewicz","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]},{"given":"B","family":"Calabretta","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107, USA."}]}],"member":"341","published-online":{"date-parts":[[1995,8]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.16.7435","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T18:08:20Z","timestamp":1649873300000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.16.7435"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8]]},"references-count":0,"journal-issue":{"issue":"16","published-print":{"date-parts":[[1995,8]]}},"alternative-id":["10.1073\/pnas.92.16.7435"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.16.7435","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,8]]},"assertion":[{"value":"1995-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}