{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T06:09:41Z","timestamp":1770703781487,"version":"3.49.0"},"reference-count":0,"publisher":"Proceedings of the National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1995,8,15]]},"abstract":"<jats:p>Human melanoma cells can process the MAGE-1 gene product and present the processed nonapeptide EADPTGHSY on their major histocompatibility complex class I molecules, HLA-A1, as a determinant for cytolytic T lymphocytes (CTLs). Considering that autologous antigen presenting cells (APCs) pulsed with the synthetic nonapeptide might, therefore, be immunogenic, melanoma patients whose tumor cells express the MAGE-1 gene and who are HLA-A1+ were immunized with a vaccine made of cultured autologous APCs pulsed with the synthetic nonapeptide. Analyses of the nature of the in vivo host immune response to the vaccine revealed that the peptide-pulsed APCs are capable of inducing autologous melanoma-reactive and the nonapeptide-specific CTLs in situ at the immunization site and at distant metastatic disease sites.<\/jats:p>","DOI":"10.1073\/pnas.92.17.8078","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T13:17:17Z","timestamp":1149081437000},"page":"8078-8082","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":239,"title":["Induction of antigen-specific cytolytic T cells in situ in human melanoma by immunization with synthetic peptide-pulsed autologous antigen presenting cells."],"prefix":"10.1073","volume":"92","author":[{"given":"B","family":"Mukherji","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"N G","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"S","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"T","family":"Okino","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"H","family":"Yamase","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"J R","family":"Sporn","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"S K","family":"Kurtzman","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"M T","family":"Ergin","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"J","family":"Ozols","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]},{"given":"J","family":"Meehan","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut School of Medicine, Farmington 06030, USA."}]}],"member":"341","published-online":{"date-parts":[[1995,8,15]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.92.17.8078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T17:55:16Z","timestamp":1649872516000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.92.17.8078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8,15]]},"references-count":0,"journal-issue":{"issue":"17","published-print":{"date-parts":[[1995,8,15]]}},"alternative-id":["10.1073\/pnas.92.17.8078"],"URL":"https:\/\/doi.org\/10.1073\/pnas.92.17.8078","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,8,15]]},"assertion":[{"value":"1995-08-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}