{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:02:25Z","timestamp":1781107345236,"version":"3.54.1"},"reference-count":18,"publisher":"National Academy of Sciences","issue":"22","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,10,28]]},"abstract":"<jats:p>\n                    We cloned two hemoglobin genes from\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    . One gene,\n                    <jats:italic>AHB1<\/jats:italic>\n                    , is related in sequence to the family of nonsymbiotic hemoglobin genes previously identified in a number of plant species (class 1). The second hemoglobin gene,\n                    <jats:italic>AHB2<\/jats:italic>\n                    , represents a class of nonsymbiotic hemoglobin (class 2) related in sequence to the symbiotic hemoglobin genes of legumes and\n                    <jats:italic>Casuarina<\/jats:italic>\n                    . The properties of these two hemoglobins suggest that the two families of nonsymbiotic hemoglobins may differ in function from each other and from the symbiotic hemoglobins.\n                    <jats:italic>AHB1<\/jats:italic>\n                    is induced, in both roots and rosette leaves, by low oxygen levels. Recombinant AHB1 has an oxygen affinity so high as to make it unlikely to function as an oxygen transporter.\n                    <jats:italic>AHB2<\/jats:italic>\n                    is expressed at a low level in rosette leaves and is low temperature-inducible. AHB2 protein has a lower affinity for oxygen than AHB1 but is similar to AHB1 in having an unusually low, pH-sensitive oxygen off-rate.\n                  <\/jats:p>","DOI":"10.1073\/pnas.94.22.12230","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:35:50Z","timestamp":1027679750000},"page":"12230-12234","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":228,"title":["Two hemoglobin genes in\n                    <i>Arabidopsis thaliana<\/i>\n                    : The evolutionary origins of\u2009leghemoglobins"],"prefix":"10.1073","volume":"94","author":[{"given":"Ben","family":"Trevaskis","sequence":"first","affiliation":[{"name":"Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia; Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra, Australian Capital Territory, 2601 Australia; Department of Biochemistry and Molecular Biology, Australian National University, Australian Capital Territory, 2601 Australia; and Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard. A.","family":"Watts","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia; Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra, Australian Capital Territory, 2601 Australia; Department of Biochemistry and Molecular Biology, Australian National University, Australian Capital Territory, 2601 Australia; and Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol R.","family":"Andersson","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia; Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra, Australian Capital Territory, 2601 Australia; Department of Biochemistry and Molecular Biology, Australian National University, Australian Capital Territory, 2601 Australia; and Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny J.","family":"Llewellyn","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia; Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra, Australian Capital Territory, 2601 Australia; Department of Biochemistry and Molecular Biology, Australian National University, Australian Capital Territory, 2601 Australia; and Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Hargrove","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia; Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra, Australian Capital Territory, 2601 Australia; Department of Biochemistry and Molecular Biology, Australian National University, Australian Capital Territory, 2601 Australia; and Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John S.","family":"Olson","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia; Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra, Australian Capital Territory, 2601 Australia; Department of Biochemistry and Molecular Biology, Australian National University, Australian Capital Territory, 2601 Australia; and Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth S.","family":"Dennis","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia; Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra, Australian Capital Territory, 2601 Australia; Department of Biochemistry and Molecular Biology, Australian National University, Australian Capital Territory, 2601 Australia; and Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. James","family":"Peacock","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Plant Cell Biology, Australian National University, Australian Capital Territory, 2601 Australia; Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1600, Canberra, Australian Capital Territory, 2601 Australia; Department of Biochemistry and Molecular Biology, Australian National University, Australian Capital Territory, 2601 Australia; and Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street,..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1997,10,28]]},"reference":[{"key":"e_1_3_3_1_2","unstructured":"Kubo H. (1939) in Appleby C. A. (1992) Sci. Prog. 76 365\u2013398."},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.35.060184.002303"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(87)90010-0"},{"key":"e_1_3_3_4_2","first-page":"213","volume":"7","author":"Jacobsen-Lyon K","year":"1995","unstructured":"K Jacobsen-Lyon, E \u00d8 Jensen, J Jorgensen, K A Marcker, W J Peacock, E S Dennis Plant Cell 7, 213\u2013223 (1995).","journal-title":"Plant Cell"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/324166a0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/331178a0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5682"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00014440"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.4.1075"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90086-8"},{"key":"e_1_3_3_11_2","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel F M","year":"1994","unstructured":"F M Ausubel, R Brenton, R E Kingston, D D Moore, J G Siedman, J A Smyth, K Struhl Current Protocols in Molecular Biology (Wiley, New York, 1994)."},{"key":"e_1_3_3_12_2","first-page":"4","volume":"4","author":"Taylor A","year":"1982","unstructured":"A Taylor, B Powell BRL Focus 4, 4 (1982).","journal-title":"BRL Focus"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.24.8961"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0833"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.5.1408"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(87)90521-3"},{"key":"e_1_3_3_17_2","first-page":"847","volume":"32","author":"Millar A H","year":"1994","unstructured":"A H Millar, F J Bergerson, D A Day Plant Physiol Biochem 32, 847\u2013852 (1994).","journal-title":"Plant Physiol Biochem"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.19.060190.001245"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.94.22.12230","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T17:32:01Z","timestamp":1654536721000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.94.22.12230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10,28]]},"references-count":18,"journal-issue":{"issue":"22","published-print":{"date-parts":[[1997,10,28]]}},"alternative-id":["10.1073\/pnas.94.22.12230"],"URL":"https:\/\/doi.org\/10.1073\/pnas.94.22.12230","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10,28]]},"assertion":[{"value":"1997-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-08-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-10-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}