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This endonucleolytic processing at and close to the tRNA 3\u2032 end in potato mitochondria suggests a higher similarity to the eukaryotic than to the prokaryotic tRNA 3\u2032 processing pathway. Partial purification and separation of RNase Z from the 5\u2032 processing activity RNase P allowed us to determine biochemical characteristics of the enzyme. The activity is stable over broad pH and temperature ranges, with peak activity at pH 8 and 30\u00b0C. Optimal concentrations for MgCl\n                    <jats:sub>2<\/jats:sub>\n                    and KCl are 5 mM and 30 mM, respectively. The potato mitochondrial RNase Z accepts only tRNA precursors with mature 5\u2032 ends. The precursor for tRNA\n                    <jats:sup>Phe<\/jats:sup>\n                    requires RNA editing for efficient processing by RNase Z.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.1.108","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:35:50Z","timestamp":1027679750000},"page":"108-113","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":62,"title":["5\u2032 end maturation and RNA editing have to precede tRNA 3\u2032 processing in\u2009plant\u2009mitochondria"],"prefix":"10.1073","volume":"95","author":[{"given":"Anja","family":"Kunzmann","sequence":"first","affiliation":[{"name":"Allgemeine Botanik, Universit\u00e4t Ulm, Albert-Einstein-Allee 11, D-89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Brennicke","sequence":"additional","affiliation":[{"name":"Allgemeine Botanik, Universit\u00e4t Ulm, Albert-Einstein-Allee 11, D-89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Marchfelder","sequence":"additional","affiliation":[{"name":"Allgemeine Botanik, Universit\u00e4t Ulm, Albert-Einstein-Allee 11, D-89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1998,1,6]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(92)90262-8"},{"key":"e_1_3_3_2_2","first-page":"67","volume-title":"tRNA: Structure, Biosynthesis and Function","author":"Altman S","year":"1995","unstructured":"S Altman, L Kirsebom, S Talbot tRNA: Structure, Biosynthesis and Function, eds D S\u00f6ll, U RajBhandary (Am. 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