{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:46:06Z","timestamp":1762112766519},"reference-count":34,"publisher":"Proceedings of the National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,7,7]]},"abstract":"<jats:p>Generation and negative selection of NK1.1<jats:sup>+<\/jats:sup>\u03b1\/\u03b2 T cell receptor (TCR)<jats:sup>+<\/jats:sup>thymocytes were analyzed using TCR-transgenic (B10.D2 \u00d7 DO10)F<jats:sub>1<\/jats:sub>and (C57BL\/6 \u00d7 DO10)F<jats:sub>1<\/jats:sub>mice and Rag-1<jats:sup>\u2212\/\u2212<\/jats:sup>\/DO10 mice, which had been established by breeding and backcrossing between Rag-1<jats:sup>\u2212\/\u2212<\/jats:sup>and DO10 mice. Almost all T cells from these mice were shown to bear V\u03b113\/V\u03b28.2 that is specific for chicken ovalbumin (cOVA) and restricted to I-A<jats:sup>d<\/jats:sup>. A normal proportion of the NK1.1<jats:sup>+<\/jats:sup>V\u03b113\/V\u03b28.2<jats:sup>+<\/jats:sup>thymocytes was generated in these mice. However, the actual cell number of both NK1.1<jats:sup>+<\/jats:sup>and NK1.1<jats:sup>\u2212<\/jats:sup>thymocytes in I-A<jats:sup>d\/d<\/jats:sup>mice (positive selecting background) was larger than that in I-A<jats:sup>b\/d<\/jats:sup>mice (negative selecting background). Markedly low but significant proportions of NK1.1<jats:sup>+<\/jats:sup>V\u03b113\/V\u03b28.2<jats:sup>+<\/jats:sup>cells were detected in the spleens from I-A<jats:sup>d\/d<\/jats:sup>and I-A<jats:sup>b\/d<\/jats:sup>mice. It was shown that the splenic NK1.1<jats:sup>+<\/jats:sup>T cells of the I-A<jats:sup>b\/d<\/jats:sup>mice were anergized against stimulation through TCR. When (B10.D2 \u00d7 DO10)F<jats:sub>1<\/jats:sub>and (C57BL\/6 \u00d7 DO10)F<jats:sub>1<\/jats:sub>mice were given cOVA, extensive or intermediate elimination of NK1.1<jats:sup>+<\/jats:sup>\u03b1\/\u03b2TCR<jats:sup>+<\/jats:sup>thymocytes was induced in I-A<jats:sup>d\/d<\/jats:sup>or I-A<jats:sup>b\/d<\/jats:sup>mice, respectively. However, the clonal elimination was not as complete as that seen in the major NK1.1<jats:sup>\u2212<\/jats:sup>thymocyte population. The present findings indicate that normal generation of NK1.1<jats:sup>+<\/jats:sup>\u03b1\/\u03b2TCR<jats:sup>+<\/jats:sup>thymocytes occurs in the absence of V\u03b114-J\u03b1281 and that substantial negative selection operates on the NK1.1<jats:sup>+<\/jats:sup>\u03b1\/\u03b2TCR<jats:sup>+<\/jats:sup>cells.<\/jats:p>","DOI":"10.1073\/pnas.95.14.8199","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:35:50Z","timestamp":1027694150000},"page":"8199-8204","update-policy":"http:\/\/dx.doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":19,"title":["Intrathymic selection of NK1.1<sup>+<\/sup>\u03b1\/\u03b2 T cell antigen receptor (TCR)<sup>+<\/sup>cells in transgenic mice bearing TCR specific for chicken ovalbumin and restricted to I-A<sup>d<\/sup>"],"prefix":"10.1073","volume":"95","author":[{"given":"Chikako","family":"Iwabuchi","sequence":"first","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 33701"}]},{"given":"Kazuya","family":"Iwabuchi","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 33701"}]},{"given":"Ken-ichi","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 33701"}]},{"given":"Toshiaki","family":"Takayanagi","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 33701"}]},{"given":"Hiroki","family":"Nishihori","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 33701"}]},{"given":"Saori","family":"Tone","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 33701"}]},{"given":"Kazumasa","family":"Ogasawara","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 33701"}]},{"given":"Robert A.","family":"Good","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 33701"}]},{"given":"Kazunori","family":"Ono\u00e9","sequence":"additional","affiliation":[{"name":"Section of Pathology, Institute of Immunological Science, Hokkaido University, Sapporo 060, Japan; and Department of Pediatrics, University of South Florida\/All Children\u2019s Hospital, St. Petersburg, FL 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