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The silencing cassette is not expressed until activated by addition of CRE recombinase delivered by a lentiviral vector. We have used this system to show specific down-regulation of GFP and two endogenous genes (the tumor suppressor p53 and the NF-\u03baB transcription factor subunit p65)\n            <jats:italic>in vitro<\/jats:italic>\n            . Furthermore, down-regulation of both p53 and p65 resulted in the expected effect on downstream genes and cellular phenotype. We foresee multiple applications of this system both\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            to down-regulate specific targets in a tissue-specific and localized manner.\n          <\/jats:p>","DOI":"10.1073\/pnas.0402107101","type":"journal-article","created":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T01:13:19Z","timestamp":1083373999000},"page":"7347-7351","update-policy":"http:\/\/dx.doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":111,"title":["CRE recombinase-inducible RNA interference mediated by lentiviral vectors"],"prefix":"10.1073","volume":"101","author":[{"given":"Gustavo","family":"Tiscornia","sequence":"first","affiliation":[{"name":"Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037"}]},{"given":"Vinay","family":"Tergaonkar","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037"}]},{"given":"Francesco","family":"Galimi","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037"}]},{"given":"Inder M.","family":"Verma","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037"}]}],"member":"341","published-online":{"date-parts":[[2004,4,30]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"389","volume":"5","year":"2003","unstructured":"Wilson, J. 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