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Using<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>-selective microssensors inserted into the diffusional field of<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>in acute hippocampal slices, we describe the<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>concentration dynamics evoked by NMDA receptor activation and report profound differences along the trisynaptic loop of the hippocampus. We measured the oxygen gradient across the slice thickness and conclude that<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>measurements were performed at cell layers experiencing physiological oxygen tensions. Recordings performed at increasing distances from the point of NMDA receptor stimulation resulted in a progressive decrease of<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>signals, reaching undetectable levels for distances &gt;400 \u03bcm, supporting the notion of a wide diffusional spread of endogenously generated<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>in the hippocampus. Neither a picoinjection nor a continuous perfusion of NMDA resulted in high steady-state<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>levels; rather all signals were transient, suggesting that cells are able to efficiently respond to high<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>concentrations (typically 200-400 nM) bringing it to very low nM levels; the claimed high micromolar<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>range achieved by excessive stimulation of NMDA receptor may have to be reevaluated. The distinct responses to NMDA receptor stimulation along the trysynaptic loop suggest a differential<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>activity and\/or regulation among the hippocampal subregions. These findings may be relevant for the understanding of the role of<jats:inline-formula><jats:tex-math notation=\"LaTeX\">\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} \\begin{equation*}^{{\\cdot}}{\\mathrm{N}}{\\mathrm{O}}\\end{equation*}\\end{document}<\/jats:tex-math><\/jats:inline-formula>in physiologic mechanisms in the hippocampus and the differential sensitivity of the hippocampal subregions to NMDA receptor-dependent neurodegeneration.<\/jats:p>","DOI":"10.1073\/pnas.0503624102","type":"journal-article","created":{"date-parts":[[2005,11,18]],"date-time":"2005-11-18T01:33:39Z","timestamp":1132277619000},"page":"17483-17488","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":82,"title":["Concentration dynamics of nitric oxide in rat hippocampal subregions evoked by stimulation of the NMDA glutamate receptor"],"prefix":"10.1073","volume":"102","author":[{"given":"Ana","family":"Ledo","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy and Center for Neurosciences and Cell Biology, University of Coimbra, 3000 Coimbra, Portugal; 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