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Compared with wild-type mice (Y<jats:sub>1<\/jats:sub>+\/+), the adrenal content and constitutive release of catecholamine were increased in chromaffin cells from Y<jats:sub>1<\/jats:sub>\u2212\/\u2212 mice. In resting animals, catecholamine plasma concentrations were higher in Y<jats:sub>1<\/jats:sub>\u2212\/\u2212 mice. Comparing the adrenal glands of both genotypes, no differences were observed in the area of the medulla, cortex, and X zone. The high turnover of adrenal catecholamine in Y<jats:sub>1<\/jats:sub>\u2212\/\u2212 mice was explained by the enhancement of tyrosine hydroxylase (TH) activity, although no change in the affinity of the enzyme was observed. The molecular interaction between the Y<jats:sub>1<\/jats:sub>receptor and TH was demonstrated by the fact that NPY markedly inhibited the forskolin-induced luciferin activity in Y<jats:sub>1<\/jats:sub>receptor-expressing SK-N-MC cells transfected with a TH promoter sequence. We propose that NPY controls the release and synthesis of catecholamine from the adrenal medulla and consequently contributes to the sympathoadrenal tone.<\/jats:p>","DOI":"10.1073\/pnas.0600913103","type":"journal-article","created":{"date-parts":[[2006,6,24]],"date-time":"2006-06-24T00:53:12Z","timestamp":1151110392000},"page":"10497-10502","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["Deletion of the neuropeptide Y (NPY) Y<sub>1<\/sub>receptor gene reveals a regulatory role of NPY on catecholamine synthesis and secretion"],"prefix":"10.1073","volume":"103","author":[{"given":"Cl\u00e1udia","family":"Cavadas","sequence":"first","affiliation":[{"name":"*Center for Neurosciences and Cell Biology and Faculty of Pharmacy, University of Coimbra, 3004-517 Coimbra, Portugal;"}]},{"given":"Daniel","family":"C\u00e9fai","sequence":"additional","affiliation":[{"name":"Department of Medicine, Divisions of"}]},{"given":"Joana","family":"Rosmaninho-Salgado","sequence":"additional","affiliation":[{"name":"*Center for Neurosciences and Cell Biology and Faculty of Pharmacy, University of Coimbra, 3004-517 Coimbra, Portugal;"}]},{"given":"Maria Augusta","family":"Vieira-Coelho","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Therapeutics, Faculty of Medicine, 4200-319 Porto, Portugal"}]},{"given":"Eduardo","family":"Moura","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Therapeutics, Faculty of Medicine, 4200-319 Porto, Portugal"}]},{"given":"Nathalie","family":"Busso","sequence":"additional","affiliation":[{"name":"Rheumatology,"}]},{"given":"Thierry","family":"Pedrazzini","sequence":"additional","affiliation":[{"name":"Department of Medicine, Divisions of"}]},{"given":"Daniela","family":"Grand","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Toxicology, and"}]},{"given":"Samuel","family":"Rotman","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Centre Hospitalier Universaire Vaudois, 1011 Lausanne, Switzerland; and"}]},{"given":"Bernard","family":"Waeber","sequence":"additional","affiliation":[{"name":"**Clinical Pathophysiology, and"}]},{"given":"Jean-Fran\u00e7ois","family":"Aubert","sequence":"additional","affiliation":[{"name":"Department of Medicine, Divisions of"}]},{"given":"Eric","family":"Grouzmann","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Toxicology, and"}]}],"member":"341","published-online":{"date-parts":[[2006,7,5]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(85)90085-0"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-8673.1994.tb00601.x"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0031-6997(24)01352-8","volume":"50","author":"Michel M. 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