{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:36:21Z","timestamp":1772825781670,"version":"3.50.1"},"reference-count":81,"publisher":"Proceedings of the National Academy of Sciences","issue":"18","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,5,6]]},"abstract":"<jats:p>\n            Wnt target gene transcription is mediated by nuclear translocation of stabilized \u03b2-catenin, which binds to TCF and recruits Pygopus, a cofactor with an unknown mechanism of action. The mediator complex is essential for the transcription of RNA polymerase II-dependent genes; it associates with an accessory subcomplex consisting of the Med12, Med13, Cdk8, and Cyclin C subunits. We show here that the Med12 and Med13 subunits of the\n            <jats:italic>Drosophila<\/jats:italic>\n            mediator complex, encoded by\n            <jats:italic>kohtalo<\/jats:italic>\n            and\n            <jats:italic>skuld<\/jats:italic>\n            , are essential for the transcription of Wingless target genes.\n            <jats:italic>kohtalo<\/jats:italic>\n            and\n            <jats:italic>skuld<\/jats:italic>\n            act downstream of \u03b2-catenin stabilization both\n            <jats:italic>in vivo<\/jats:italic>\n            and in cell culture. They are required for transcriptional activation by the N-terminal domain of Pygopus, and their physical interaction with Pygopus depends on this domain. We propose that Pygopus promotes Wnt target gene transcription by recruiting the mediator complex through interactions with Med12 and Med13.\n          <\/jats:p>","DOI":"10.1073\/pnas.0709749105","type":"journal-article","created":{"date-parts":[[2008,5,4]],"date-time":"2008-05-04T01:41:26Z","timestamp":1209865286000},"page":"6644-6649","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":114,"title":["Pygopus activates Wingless target gene transcription through the mediator complex subunits Med12 and Med13"],"prefix":"10.1073","volume":"105","author":[{"given":"In\u00e9s","family":"Carrera","sequence":"first","affiliation":[{"name":"Kimmel Center for Biology and Medicine of the Skirball Institute and Department of Cell Biology, New York University School of Medicine, 540 First Avenue, New York, NY 10016"}]},{"given":"Florence","family":"Janody","sequence":"additional","affiliation":[{"name":"Kimmel Center for Biology and Medicine of 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