{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T21:12:10Z","timestamp":1771017130883,"version":"3.50.1"},"reference-count":27,"publisher":"Proceedings of the National Academy of Sciences","issue":"22","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,6,2]]},"abstract":"<jats:p>\n            Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of\n            <jats:italic>Oct4<\/jats:italic>\n            ,\n            <jats:italic>Sox2<\/jats:italic>\n            ,\n            <jats:italic>Klf4<\/jats:italic>\n            ,\n            <jats:italic>c-Myc<\/jats:italic>\n            ,\n            <jats:italic>Nanog<\/jats:italic>\n            , and\n            <jats:italic>Lin28<\/jats:italic>\n            . The delivery of these transcription factors has mostly entailed the use of integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing\n            <jats:italic>Oct4<\/jats:italic>\n            ,\n            <jats:italic>Sox2<\/jats:italic>\n            ,\n            <jats:italic>Klf4<\/jats:italic>\n            , and\n            <jats:italic>c-Myc<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1073\/pnas.0901471106","type":"journal-article","created":{"date-parts":[[2009,5,20]],"date-time":"2009-05-20T02:29:01Z","timestamp":1242786541000},"page":"8918-8922","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":193,"title":["Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector"],"prefix":"10.1073","volume":"106","author":[{"given":"Federico","family":"Gonzalez","sequence":"first","affiliation":[{"name":"Center of Regenerative Medicine of Barcelona, Dr. Aiguader 88, p7, Barcelona 08003, Spain; and"}]},{"given":"Montserrat","family":"Barragan Monasterio","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine of Barcelona, Dr. Aiguader 88, p7, Barcelona 08003, Spain; and"}]},{"given":"Gustavo","family":"Tiscornia","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine of Barcelona, Dr. Aiguader 88, p7, Barcelona 08003, Spain; and"}]},{"given":"Nuria","family":"Montserrat Pulido","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine of Barcelona, Dr. Aiguader 88, p7, Barcelona 08003, Spain; and"}]},{"given":"Rita","family":"Vassena","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine of Barcelona, Dr. Aiguader 88, p7, Barcelona 08003, Spain; and"}]},{"given":"Laura","family":"Batlle Morera","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine of Barcelona, Dr. Aiguader 88, p7, Barcelona 08003, Spain; and"}]},{"given":"Ignasi","family":"Rodriguez Piza","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine of Barcelona, Dr. Aiguader 88, p7, Barcelona 08003, Spain; and"}]},{"given":"Juan Carlos Izpisua","family":"Belmonte","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine of Barcelona, Dr. Aiguader 88, p7, Barcelona 08003, Spain; and"},{"name":"The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037"}]}],"member":"341","published-online":{"date-parts":[[2009,6,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.024"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.05.014"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05934"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05944"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711983105"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1154884"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162494"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.05.010"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.03.028"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07061"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0317"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1503"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.08.003"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151526"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1374"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.12.001"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1502"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.10.004"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.01.004"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.02.001"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.08.008"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.08.014"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164270"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06337.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0813"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811426106"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0050"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0901471106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T22:22:57Z","timestamp":1649802177000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0901471106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6,2]]},"references-count":27,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2009,6,2]]}},"alternative-id":["10.1073\/pnas.0901471106"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0901471106","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6,2]]},"assertion":[{"value":"2008-12-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}