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Here we describe the role of two AGO-class paralogs,\n            <jats:italic>alg-3<\/jats:italic>\n            (T22B3.2) and\n            <jats:italic>alg-4<\/jats:italic>\n            (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP::\n            <jats:italic>alg-3<\/jats:italic>\n            transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis.\n            <jats:italic>alg-3<\/jats:italic>\n            \/\n            <jats:italic>4<\/jats:italic>\n            double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs. These findings add to a growing number of AGO pathways required for thermotolerant fertility in\n            <jats:italic>C. elegans<\/jats:italic>\n            and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks.\n          <\/jats:p>","DOI":"10.1073\/pnas.0911685107","type":"journal-article","created":{"date-parts":[[2010,2,5]],"date-time":"2010-02-05T03:34:56Z","timestamp":1265340896000},"page":"3588-3593","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":232,"title":["Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in\n            <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1073","volume":"107","author":[{"given":"Colin C.","family":"Conine","sequence":"first","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;"}]},{"given":"Pedro J.","family":"Batista","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;"},{"name":"Gulbenkian PhD Programme in Biomedicine, Rua da Quinta Grande, 6, 2780-156 Oeiras, Portugal; and"}]},{"given":"Weifeng","family":"Gu","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;"}]},{"given":"Julie M.","family":"Claycomb","sequence":"additional","affiliation":[{"name":"The Howard Hughes Medical Institute, and"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;"}]},{"given":"Daniel A.","family":"Chaves","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;"},{"name":"Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisbon, Portugal"}]},{"given":"Masaki","family":"Shirayama","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;"}]},{"given":"Craig C.","family":"Mello","sequence":"additional","affiliation":[{"name":"The Howard Hughes Medical Institute, and"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606;"}]}],"member":"341","published-online":{"date-parts":[[2010,2,23]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/nrm2321","article-title":"Argonaute proteins: key players in RNA silencing","volume":"9","author":"Hutvagner G","year":"2008","unstructured":"G Hutvagner, MJ Simard, Argonaute proteins: key players in RNA silencing. 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Cell 106, 23\u201334 (2001).","journal-title":"Cell"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.cell.2009.09.014","article-title":"The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation","volume":"139","author":"Claycomb JM","year":"2009","unstructured":"JM Claycomb, et al., The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation. Cell 139, 123\u2013134 (2009).","journal-title":"Cell"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.molcel.2008.06.002","article-title":"PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans","volume":"31","author":"Batista PJ","year":"2008","unstructured":"PJ Batista, et al., PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans. Mol Cell 31, 67\u201378 (2008).","journal-title":"Mol Cell"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.molcel.2008.06.003","article-title":"Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline","volume":"31","author":"Das PP","year":"2008","unstructured":"PP Das, et al., Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline. Mol Cell 31, 79\u201390 (2008).","journal-title":"Mol Cell"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.cub.2008.05.009","article-title":"A C. elegans Piwi, PRG-1, regulates 21U-RNAs during spermatogenesis","volume":"18","author":"Wang G","year":"2008","unstructured":"G Wang, V Reinke, A C. elegans Piwi, PRG-1, regulates 21U-RNAs during spermatogenesis. 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