{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T16:43:12Z","timestamp":1771605792105,"version":"3.50.1"},"reference-count":30,"publisher":"Proceedings of the National Academy of Sciences","issue":"51","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2011,12,20]]},"abstract":"<jats:p>Cyclic nucleotide-regulated ion channels are present in bacteria, plants, vertebrates, and humans. In higher organisms, they are closely involved in signaling networks of vision and olfaction. Binding of cAMP or cGMP favors the activation of these ion channels. Despite a wealth of structural and studies, there is a lack of structural data describing the gating process in a full-length cyclic nucleotide-regulated channel. We used high-resolution atomic force microscopy (AFM) to directly observe the conformational change of the membrane embedded bacterial cyclic nucleotide-regulated channel MlotiK1. In the nucleotide-bound conformation, the cytoplasmic cyclic nucleotide-binding (CNB) domains of MlotiK1 are disposed in a fourfold symmetric arrangement forming a pore-like vestibule. Upon nucleotide-unbinding, the four CNB domains undergo a large rearrangement, stand up by \u223c1.7 nm, and adopt a structurally variable grouped conformation that closes the cytoplasmic vestibule. This fully reversible conformational change provides insight into how CNB domains rearrange when regulating the potassium channel.<\/jats:p>","DOI":"10.1073\/pnas.1111149108","type":"journal-article","created":{"date-parts":[[2011,12,3]],"date-time":"2011-12-03T06:06:49Z","timestamp":1322892409000},"page":"20802-20807","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":48,"title":["Gating of the MlotiK1 potassium channel involves large rearrangements of the cyclic nucleotide-binding domains"],"prefix":"10.1073","volume":"108","author":[{"given":"Stefania A.","family":"Mari","sequence":"first","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland;"}]},{"given":"Jo\u00e3o","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal;"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Largo Professor Abel Salazar 2, 4150-003 Porto, Portugal;"}]},{"given":"Stephen","family":"Altieri","sequence":"additional","affiliation":[{"name":"Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06517; and"}]},{"given":"Ulf","family":"Hensen","sequence":"additional","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland;"}]},{"given":"Lise","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Quinnipiac University, Hamden, CT 06517"}]},{"given":"Jo\u00e3o H.","family":"Morais-Cabral","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal;"}]},{"given":"Daniel J.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland;"}]}],"member":"341","published-online":{"date-parts":[[2011,12]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.63.1.235"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00008.2002"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.19.110701.154854"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.10.030"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.06.011"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1015890108"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104607"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0908380106"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1341"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711533105"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2009.06.012"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2007.06.020"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.309"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2008.09.046"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/78929"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2008.100"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf365"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M609317200"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.12.034"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00206-7"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2009.04.011"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00623-1"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7401025"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)00065-4"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.166"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1997.3875"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2359"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3991(79)80044-3"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1982.tb00405.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1988.tb04602.x"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1111149108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:39:16Z","timestamp":1649810356000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1111149108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":30,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2011,12,20]]}},"alternative-id":["10.1073\/pnas.1111149108"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1111149108","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12]]},"assertion":[{"value":"2011-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}