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We obtained comprehensive geochemical data from two gravity cores near the hydrothermal vent field Loki\u2019s Castle at the Arctic Mid-Ocean Ridge, in the Norwegian-Greenland Sea. Geochemical properties in the rift valley sediments exhibited strong centimeter-scale stratigraphic variability. Microbial populations were profiled by pyrosequencing from 15 sediment horizons (59,364 16S rRNA gene tags), quantitatively assessed by qPCR, and phylogenetically analyzed. Although the same taxa were generally present in all samples, their relative abundances varied substantially among horizons and fluctuated between Bacteria- and Archaea-dominated communities. By independently summarizing covariance structures of the relative abundance data and geochemical data, using principal components analysis, we found a significant correlation between changes in geochemical composition and changes in community structure. Differences in organic carbon and mineralogy shaped the relative abundance of microbial taxa. We used correlations to build hypotheses about energy metabolisms, particularly of the Deep Sea Archaeal Group, specific Deltaproteobacteria, and sediment lineages of potentially anaerobic Marine Group I Archaea. We demonstrate that total prokaryotic community structure can be directly correlated to geochemistry within these sediments, thus enhancing our understanding of biogeochemical cycling and our ability to predict metabolisms of uncultured microbes in deep-sea sediments.<\/jats:p>","DOI":"10.1073\/pnas.1207574109","type":"journal-article","created":{"date-parts":[[2012,10,2]],"date-time":"2012-10-02T06:37:49Z","timestamp":1349159869000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":254,"title":["Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge"],"prefix":"10.1073","volume":"109","author":[{"given":"Steffen Leth","family":"Jorgensen","sequence":"first","affiliation":[{"name":"Centre for Geobiology, Department of Biology, and"}]},{"given":"Bjarte","family":"Hannisdal","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Earth Science, University of Bergen, 5007 Bergen, Norway;"}]},{"given":"Anders","family":"Lanz\u00e9n","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Biology, and"},{"name":"Computational Biology Unit, Uni Computing, Uni Research, 5007 Bergen, Norway;"}]},{"given":"Tamara","family":"Baumberger","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Earth Science, University of Bergen, 5007 Bergen, Norway;"},{"name":"Institute for Geochemistry and Petrology, Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich, 8092 Zurich, Switzerland;"}]},{"given":"Kristin","family":"Flesland","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Earth Science, University of Bergen, 5007 Bergen, Norway;"}]},{"given":"Rita","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Geosciences, University of \u00c9vora, 7000 \u00c9vora, Portugal;"},{"name":"Creminer Laboratory of Robotics and Systems in Engineering Science (LARSyS), Faculty of Sciences, University of Lisbon, 1749-016 Lisboa, Portugal; and"}]},{"given":"Lise","family":"\u00d8vre\u00e5s","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Biology, and"}]},{"given":"Ida H.","family":"Steen","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Biology, and"}]},{"given":"Ingunn H.","family":"Thorseth","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Earth Science, University of Bergen, 5007 Bergen, Norway;"}]},{"given":"Rolf B.","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Earth Science, University of Bergen, 5007 Bergen, Norway;"}]},{"given":"Christa","family":"Schleper","sequence":"additional","affiliation":[{"name":"Centre for Geobiology, Department of Biology, and"},{"name":"Department of Genetics in Ecology, University of Vienna, A-1090 Vienna, Austria"}]}],"member":"341","published-online":{"date-parts":[[2012,10]]},"reference":[{"key":"e_1_1_2_18_10_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03796"},{"key":"e_1_1_2_18_10_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6578"},{"key":"e_1_1_2_18_10_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1124"},{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"6578","DOI":"10.1073\/pnas.95.12.6578","article-title":"Prokaryotes: The unseen majority","volume":"95","author":"Whitman WB","year":"1998","unstructured":"WB Whitman, DC Coleman, WJ Wiebe, Prokaryotes: The unseen majority. 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