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Depending on its ligation status, IscR recognizes and binds to distinct promoters, therefore modulating cluster biosynthesis. This unique protein at the crossroad between the ISC and sulfur assimilation (SUF) iron-sulfur cluster biosynthetic pathways was thought to be restricted to Gram-negative bacteria. We demonstrated the existence of a functional IscR in the unique Gram-positive bacterium\n            <jats:italic>Thermincola potens<\/jats:italic>\n            . Structural and functional analysis of\n            <jats:italic>T. potens<\/jats:italic>\n            and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            IscR unveiled a conserved mechanism of promoter discrimination, along with subtle structural differences that explain their distinct DNA sequence recognition specificity.\n          <\/jats:p>","DOI":"10.1073\/pnas.1322728111","type":"journal-article","created":{"date-parts":[[2014,5,21]],"date-time":"2014-05-21T01:53:54Z","timestamp":1400637234000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":40,"title":["The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium"],"prefix":"10.1073","volume":"111","author":[{"given":"Joana A.","family":"Santos","sequence":"first","affiliation":[{"name":"Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4150-180 Porto, Portugal; and"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto, 4050-313 Porto, Portugal"}]},{"given":"Noelia","family":"Alonso-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4150-180 Porto, Portugal; and"}]},{"given":"Sandra","family":"Macedo-Ribeiro","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4150-180 Porto, Portugal; and"}]},{"given":"Pedro Jos\u00e9 Barbosa","family":"Pereira","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4150-180 Porto, Portugal; and"}]}],"member":"341","published-online":{"date-parts":[[2014,5,20]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1126\/science.277.5326.653","article-title":"Iron-sulfur clusters: Nature\u2019s modular, multipurpose structures","volume":"277","author":"Beinert H","year":"1997","unstructured":"H Beinert, RH Holm, E M\u00fcnck, Iron-sulfur clusters: Nature\u2019s modular, multipurpose structures. 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