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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,2,17]]},"abstract":"<jats:title>Significance<\/jats:title><jats:p>Peroxiredoxins (Prxs) are highly abundant proteins, which serve two seemingly mutually exclusive roles as peroxidases and molecular chaperones. Little is known about the precise mechanism of Prxs\u2019 activation as chaperone and the physiological significance of this second function. Here we demonstrate that in<jats:italic>Leishmania infantum<\/jats:italic>, reduced Prx provides a crucial, stress-specific chaperone reservoir, which is activated rapidly upon exposure to unfolding stress conditions. Once activated, Prx protects a wide range of different clients against protein unfolding. Clients are bound in the center of the decameric ring, providing experimental evidence for previous claims that Prxs serve as likely ancestors of chaperonins. Interference with client binding impairs<jats:italic>Leishmania<\/jats:italic>infectivity, providing compelling evidence for the in vivo importance of Prx\u2019s chaperone function.<\/jats:p>","DOI":"10.1073\/pnas.1419682112","type":"journal-article","created":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T04:18:45Z","timestamp":1422937125000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":83,"title":["Mitochondrial peroxiredoxin functions as crucial chaperone reservoir in<i>Leishmania infantum<\/i>"],"prefix":"10.1073","volume":"112","author":[{"given":"Filipa","family":"Teixeira","sequence":"first","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200 Porto, Portugal;"},{"name":"IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal;"},{"name":"ICBAS \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal;"},{"name":"Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Castro","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200 Porto, Portugal;"},{"name":"IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e2nia","family":"Cruz","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200 Porto, Portugal;"},{"name":"IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Tse","sequence":"additional","affiliation":[{"name":"Life Science Institute, University of Michigan, Ann Arbor, MI 48109; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Koldewey","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Southworth","sequence":"additional","affiliation":[{"name":"Life Science Institute, University of Michigan, Ann Arbor, MI 48109; and"},{"name":"Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Tom\u00e1s","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200 Porto, Portugal;"},{"name":"IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal;"},{"name":"ICBAS \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Jakob","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109;"},{"name":"Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,2,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.06985.x"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47038-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2002.040"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3393"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"7.9","DOI":"10.1002\/0471140856.tx0709s49","article-title":"Overview of peroxiredoxins in oxidant defense and redox regulation","volume":"49","author":"Poole LB","year":"2011","unstructured":"LB Poole, A Hall, KJ Nelson, Overview of peroxiredoxins in oxidant defense and redox regulation. 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