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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,6,9]]},"abstract":"<jats:title>Significance<\/jats:title>\n          <jats:p>Fetal extravillous trophoblasts (EVT) invade uterine tissue and interact with maternal immune cells during pregnancy. EVT express human leukocyte antigen-C (HLA-C) and -G (HLA-G). Although polymorphic HLA-C can elicit a maternal immune response, HLA-G has been associated with induction of immune tolerance. We have succeeded in isolating all maternal immune cell types as well as EVT from human placental tissue. These methods were used to elucidate the unique charateristics of EVT as well as their interaction with maternal immune cells. We demonstrate that EVT are specialized cells whose properties are not imitated by HLA\u2010G\u2013expressing surrogate cell lines. Studies using primary EVT are crucial for understanding maternal\u2013fetal tolerance and development of pregnancy complications such as preeclampsia and miscarriages.<\/jats:p>","DOI":"10.1073\/pnas.1507977112","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T14:35:29Z","timestamp":1432737329000},"page":"7219-7224","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":180,"title":["Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes"],"prefix":"10.1073","volume":"112","author":[{"given":"Tamara","family":"Tilburgs","sequence":"first","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138;"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6731-8190","authenticated-orcid":false,"given":"\u00c2ngela C.","family":"Crespo","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138;"},{"name":"PhD Program in Experimental Biology and Biomedicine, Center for Neurosciences and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal;"}]},{"given":"Anita","family":"van der Zwan","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138;"}]},{"given":"Basya","family":"Rybalov","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138;"}]},{"given":"Towfique","family":"Raj","sequence":"additional","affiliation":[{"name":"Department of Neurology, Brigham and Women\u2019s Hospital, Boston, MA 02115;"}]},{"given":"Barbara","family":"Stranger","sequence":"additional","affiliation":[{"name":"Institute for Genomics and Systems Biology, University of Chicago, Chicago, IL 60637;"}]},{"given":"Lucy","family":"Gardner","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom"}]},{"given":"Ashley","family":"Moffett","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom"}]},{"given":"Jack L.","family":"Strominger","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138;"}]}],"member":"341","published-online":{"date-parts":[[2015,5,26]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1038\/nri1897","article-title":"Immunology of placentation in eutherian mammals","volume":"6","author":"Moffett A","year":"2006","unstructured":"A Moffett, C Loke, Immunology of placentation in eutherian mammals. 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