{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:08:21Z","timestamp":1768284501906,"version":"3.49.0"},"reference-count":54,"publisher":"Proceedings of the National Academy of Sciences","issue":"8","license":[{"start":{"date-parts":[[2019,8,4]],"date-time":"2019-08-04T00:00:00Z","timestamp":1564876800000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,2,19]]},"abstract":"<jats:p>\n            Transcription termination is a critical step in the control of gene expression. One of the major termination mechanisms is mediated by Rho factor that dissociates the complex mRNA-DNA-RNA polymerase upon binding with RNA polymerase. Rho promotes termination at the end of operons, but it can also terminate transcription within leader regions, performing regulatory functions and avoiding pervasive transcription. Transcription of\n            <jats:italic>rho<\/jats:italic>\n            is autoregulated through a Rho-dependent attenuation in the leader region of the transcript. In this study, we have included an additional player in this pathway. By performing MS2-affinity purification coupled with RNA sequencing (MAPS),\n            <jats:italic>rho<\/jats:italic>\n            transcript was shown to directly interact with the small noncoding RNA SraL. Using bioinformatic in vivo and in vitro experimental analyses, SraL was shown to base pair with the 5\u2032-UTR of\n            <jats:italic>rho<\/jats:italic>\n            mRNA upregulating its expression in several growth conditions. This base pairing was shown to prevent the action of Rho over its own message. Moreover, the results obtained indicate that both ProQ and Hfq are associated with this regulation. We propose a model that contemplates the action of\n            <jats:italic>Salmonella<\/jats:italic>\n            SraL sRNA in the protection of\n            <jats:italic>rho<\/jats:italic>\n            mRNA from premature transcription termination by Rho. Note that since the interaction region between both RNAs corresponds to a very-well-conserved sequence, it is plausible to admit that this regulation also occurs in other enterobacteria.\n          <\/jats:p>","DOI":"10.1073\/pnas.1811589116","type":"journal-article","created":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T01:36:50Z","timestamp":1549330610000},"page":"3042-3051","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["SraL sRNA interaction regulates the terminator by preventing premature transcription termination of\n            <i>rho<\/i>\n            mRNA"],"prefix":"10.1073","volume":"116","author":[{"given":"In\u00eas Jesus","family":"Silva","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal;"}]},{"given":"Susana","family":"Barahona","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal;"}]},{"given":"Alex","family":"Eyraud","sequence":"additional","affiliation":[{"name":"RNA Group, Department of Biochemistry, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1E 4K8, Canada;"}]},{"given":"David","family":"Lalaouna","sequence":"additional","affiliation":[{"name":"RNA Group, Department of Biochemistry, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1E 4K8, Canada;"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7552-0225","authenticated-orcid":false,"given":"Nara","family":"Figueroa-Bossi","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), Commissariat \u00e0 l\u2019\u00e9nergie atomique, CNRS, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, 91198 Gif-sur-Yvette, France"}]},{"given":"Eric","family":"Mass\u00e9","sequence":"additional","affiliation":[{"name":"RNA Group, Department of Biochemistry, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1E 4K8, Canada;"}]},{"given":"Cec\u00edlia Maria","family":"Arraiano","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal;"}]}],"member":"341","published-online":{"date-parts":[[2019,2,4]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1146\/annurev-biochem-060815-014844","article-title":"Mechanisms of bacterial transcription termination: All good things must end","volume":"85","author":"Ray-Soni A","year":"2016","unstructured":"A Ray-Soni, MJ Bellecourt, R Landick, Mechanisms of bacterial transcription termination: All good things must end. 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