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The specificity of A-minor interactions is fundamental to RNA tertiary structure formation, as well as to their proposed role in translational accuracy. To investigate A-minor motif specificity, we analyzed mutations in an A-minor interaction within the\n                    <jats:italic>Tetrahymena<\/jats:italic>\n                    group I self-splicing intron. Thermodynamic and x-ray crystallographic results show that the A-minor interaction strongly prefers canonical base pairs over base mismatches in the receptor helix, enabling RNA interhelical packing through specific recognition of Watson\u2013Crick minor groove geometry.\n                  <\/jats:p>","DOI":"10.1073\/pnas.182221799","type":"journal-article","created":{"date-parts":[[2002,9,3]],"date-time":"2002-09-03T15:58:55Z","timestamp":1031068735000},"page":"11676-11681","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":91,"title":["Specificity of RNA\u2013RNA helix recognition"],"prefix":"10.1073","volume":"99","author":[{"given":"Daniel J.","family":"Battle","sequence":"first","affiliation":[{"name":"Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520; and Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A.","family":"Doudna","sequence":"additional","affiliation":[{"name":"Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520; and Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2002,8,20]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1146\/annurev.bb.04.060175.002401","volume":"4","author":"Sigler P B","year":"1975","unstructured":"P B Sigler Annu Rev Biophys Bioeng 4, 477\u2013527 (1975).","journal-title":"Annu Rev Biophys Bioeng"},{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1006\/jmbi.1999.3001","volume":"293","author":"Tinoco I","year":"1999","unstructured":"I Tinoco, C Bustamante J Mol Biol 293, 271\u2013281 (1999).","journal-title":"J Mol Biol"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/86221","volume":"8","author":"Doherty E A","year":"2001","unstructured":"E A Doherty, R T Batey, B Masquida, J A Doudna Nat Struct Biol 8, 339\u2013343 (2001).","journal-title":"Nat Struct Biol"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"4899","DOI":"10.1073\/pnas.081082398","volume":"98","author":"Nissen P","year":"2001","unstructured":"P Nissen, J A Ippolito, N Ban, P B Moore, T A Steitz Proc Natl Acad Sci USA 98, 4899\u20134903 (2001).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/0022-2836(71)90324-X","volume":"55","author":"Lee B","year":"1971","unstructured":"B Lee, F M Richards J Mol Biol 55, 379\u2013400 (1971).","journal-title":"J Mol Biol"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","volume":"54","author":"Brunger A T","year":"1998","unstructured":"A T Brunger, P D Adams, G M Clore, W L DeLano, P Gros, R W Grosse-Kunstleve, J S Jiang, J Kuszewski, M Nilges, N S Pannu, et al. 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