{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:43:11Z","timestamp":1774690991214,"version":"3.50.1"},"reference-count":47,"publisher":"Proceedings of the National Academy of Sciences","issue":"14","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Ministry of Education and Science | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIA-BQM\/29863\/2017"],"award-info":[{"award-number":["PTDC\/BIA-BQM\/29863\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007472","name":"Funda\u00e7\u00e3o Luso-Americana para o Desenvolvimento","doi-asserted-by":"publisher","award":["FLAD Life Science 2020"],"award-info":[{"award-number":["FLAD Life Science 2020"]}],"id":[{"id":"10.13039\/501100007472","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,4,6]]},"abstract":"<jats:title>Significance<\/jats:title>\n          <jats:p>The importance of dinucleotides in the control of bacterial physiology is still being uncovered. One of these molecules is c-di-AMP, thought to control the mechanisms of import and export of potassium ions in many bacteria, including pathogens. Maintaining a balanced internal concentration of potassium is crucial for bacterial viability, even during infection. However, there has been a lack of data showing that c-di-AMP effectively changes the activity of potassium transporters. Here, we demonstrate that c-di-AMP increases the activity levels of a potassium exporter and that pH increases the sensitivity of the transporter to c-di-AMP. Our quantitative data support, therefore, the central role of c-di-AMP in the control of the potassium homeostasis machinery in bacteria.<\/jats:p>","DOI":"10.1073\/pnas.2020653118","type":"journal-article","created":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T20:05:18Z","timestamp":1617221118000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":38,"title":["c-di-AMP, a likely master regulator of bacterial K\n            <sup>+<\/sup>\n            homeostasis machinery, activates a K\n            <sup>+<\/sup>\n            exporter"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7176-0205","authenticated-orcid":false,"given":"Tatiana B.","family":"Cereija","sequence":"first","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal;"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), Universidade do Porto, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5441-9446","authenticated-orcid":false,"given":"Jo\u00e3o P. L.","family":"Guerra","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal;"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), Universidade do Porto, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7075-5589","authenticated-orcid":false,"given":"Jo\u00e3o M. P.","family":"Jorge","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal;"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), Universidade do Porto, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4461-9716","authenticated-orcid":false,"given":"Jo\u00e3o H.","family":"Morais-Cabral","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal;"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S), Universidade do Porto, 4200-135 Porto, Portugal"}]}],"member":"341","published-online":{"date-parts":[[2021,3,31]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2019.12.004"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2337"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25102462"},{"key":"e_1_3_4_4_2","first-page":"e00462-18","article-title":"Making and breaking of an essential poison: The cyclases and phosphodiesterases that produce and degrade the essential second messenger cyclic di-AMP in bacteria","volume":"201","author":"Commichau F. M.","year":"2018","unstructured":"F. M. Commichau, J. L. Heidemann, R. Ficner, J. St\u00fclke, Making and breaking of an essential poison: The cyclases and phosphodiesterases that produce and degrade the essential second messenger cyclic di-AMP in bacteria. J. Bacteriol. 201, e00462-18 (2018).","journal-title":"J. Bacteriol."},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2015.12.007"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes8080197"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13026"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-020518-115943"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.07.046"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07953.x"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002217"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13277"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00804"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.03.039"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069425"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-017-0734-3"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aal3011"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00861-17"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00045-18"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10409-4"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.147.3.820-826.1981"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1980691"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb09148.x"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.49.2.221"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-136-12-2527"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007574"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007342"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.818716"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00028-19"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.008774"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1363"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b00633"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2019.02.002"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.641340"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300595110"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00480-15"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00959-5"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703709104"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2020.126557"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12489"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(86)25028-4"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99161-1"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2013.12.007"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01041-13"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2011.77"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(79)56026-1"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2020653118","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2020653118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:34:50Z","timestamp":1649846090000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2020653118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,31]]},"references-count":47,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2021,4,6]]}},"alternative-id":["10.1073\/pnas.2020653118"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2020653118","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,31]]},"assertion":[{"value":"2021-03-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2020653118"}}