{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T02:14:28Z","timestamp":1785204868022,"version":"3.55.0"},"reference-count":102,"publisher":"National Academy of Sciences","issue":"3","license":[{"start":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T00:00:00Z","timestamp":1704758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DEB #2114785"],"award-info":[{"award-number":["DEB #2114785"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,1,16]]},"abstract":"<jats:p>\n                    Psychoactive mushrooms in the genus\n                    <jats:italic>Psilocybe<\/jats:italic>\n                    have immense cultural value and have been used for centuries in Mesoamerica. Despite the recent surge of interest in these mushrooms due to the psychotherapeutic potential of their natural alkaloid psilocybin, their phylogeny and taxonomy remain substantially incomplete. Moreover, the recent elucidation of the psilocybin biosynthetic gene cluster is known for only five of ~165 species of\n                    <jats:italic>Psilocybe<\/jats:italic>\n                    , four of which belong to only one of two major clades. We set out to improve the phylogeny of\n                    <jats:italic>Psilocybe<\/jats:italic>\n                    using shotgun sequencing of fungarium specimens, from which we obtained 71 metagenomes including from 23 types, and conducting phylogenomic analysis of 2,983 single-copy gene families to generate a fully supported phylogeny. Molecular clock analysis suggests the stem lineage of\n                    <jats:italic>Psilocybe<\/jats:italic>\n                    arose ~67 mya and diversified ~56 mya. We also show that psilocybin biosynthesis first arose in\n                    <jats:italic>Psilocybe<\/jats:italic>\n                    , with 4 to 5 possible horizontal transfers to other mushrooms between 40 and 9 mya. Moreover, predicted orthologs of the psilocybin biosynthetic genes revealed two distinct gene orders within the biosynthetic gene cluster that corresponds to a deep split within the genus, possibly a signature of two independent acquisitions of the cluster within\n                    <jats:italic>Psilocybe<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.2311245121","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T13:46:43Z","timestamp":1704808003000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":31,"title":["Phylogenomics of the psychoactive mushroom genus\n                    <i>Psilocybe<\/i>\n                    and evolution of the psilocybin biosynthetic gene cluster"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6261-621X","authenticated-orcid":false,"given":"Alexander J.","family":"Bradshaw","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, UT 84112"},{"name":"Natural History Museum of Utah, Collections and Research, University of Utah, Salt Lake City, UT 84108"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5197-1304","authenticated-orcid":false,"given":"Virginia","family":"Ram\u00edrez-Cruz","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Humanidades, Ciencias y Tecnolog\u00edas (CONAHCYT), Departamento de Bot\u00e1nica y Zoolog\u00eda, Universidad de Guadalajara, Zapopan 45147, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali R.","family":"Awan","sequence":"additional","affiliation":[{"name":"Genomics Innovation Unit, Guy\u2019s and St.Thomas\u2019 NHS Foundation Trust, St Thomas\u2019 Hospital, London SE1 7EH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"Furci","sequence":"additional","affiliation":[{"name":"Fungi Foundation, Brooklyn, NY 11216"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4390-3678","authenticated-orcid":false,"given":"Laura","family":"Guzm\u00e1n-D\u00e1valos","sequence":"additional","affiliation":[{"name":"Departamento de Bot\u00e1nica y Zoolog\u00eda, Universidad de Guadalajara, Zapopan 45147, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7965-4389","authenticated-orcid":false,"given":"Bryn T. M.","family":"Dentinger","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, UT 84112"},{"name":"Natural History Museum of Utah, Collections and Research, University of Utah, Salt Lake City, UT 84108"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,1,9]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnhum.2014.00020"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13311-017-0542-y"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2018.11.010"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","unstructured":"E. Fries Observationes Mycologicae (Havniae: Sumptibus G. Bonnieri 2021) pp. 1815-1818.","DOI":"10.5962\/bhl.title.112534"},{"key":"e_1_3_4_5_2","volume-title":"Les Champignons Hallucinog\u00e8nes du Mexique: \u00c8tudes Ethnologiques, Taxinomiques, Biologiques, Physiologiques et Chimiques","author":"Heim R.","year":"1959","unstructured":"R. Heim, R. G. Wasson, Les Champignons Hallucinog\u00e8nes du Mexique: \u00c8tudes Ethnologiques, Taxinomiques, Biologiques, Physiologiques et Chimiques (Museum National d\u2019Histoire Naturelle, 1959)."},{"key":"e_1_3_4_6_2","unstructured":"G. Guzm\u00e1n Huerta Nueva localidad de importancia etnomicol\u00f3gica de los hongos neurotr\u00f3picos mexicanos (Necaxa Puebla) Ciencia (M\u00e9xico) 20 85\u201388 (1960)."},{"key":"e_1_3_4_7_2","volume-title":"Mar\u00eda Sabina: Mujer Esp\u00edritu [Woman Spirit]","author":"Echevarria N.","year":"2006","unstructured":"N. Echevarria, B. Arochi, M. Sabina, A. Henestrosa, A. Estrada, Mar\u00eda Sabina: Mujer Esp\u00edritu [Woman Spirit] (CONACULTA\u2013IMCINE, 2006)."},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12231-008-9033-8"},{"key":"e_1_3_4_9_2","unstructured":"J. Kristinsson Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids (Nordic Council of Ministers 2008)."},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1139\/cjb-2013-0070"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01498-22"},{"key":"e_1_3_4_12_2","first-page":"14","article-title":"Los hongos sagrados del g\u00e9nero Psilocybe en Jalisco","volume":"12","author":"Castro Jauregui O. S.","year":"2022","unstructured":"O. S. Castro Jauregui, V. Ram\u00edrez-Cruz, A. J. Bradshaw, A. Cort\u00e9s-P\u00e9rez, L. Guzm\u00e1n-D\u00e1valos, Los hongos sagrados del g\u00e9nero Psilocybe en Jalisco. Nubes y Ciencia 12, 14\u201321 (2022).","journal-title":"Nubes y Ciencia"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2022.01.003"},{"key":"e_1_3_4_14_2","first-page":"109","article-title":"Seeking the magic mushroom","volume":"49","author":"Wasson R. G.","year":"1957","unstructured":"R. G. Wasson, Seeking the magic mushroom. Life 49, 109\u2013120 (1957).","journal-title":"Life"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201705489"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2019.07.006"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1897"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0121-1"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00294.x"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0114-3"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1225473"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba0721"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-022-01026-2"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","unstructured":"A. R. Awan Convergent evolution of psilocybin biosynthesis by psychedelic mushrooms. bioRxiv [Preprint] (2018). https:\/\/doi.org\/10.1101\/374199 (Accessed 15 November 2023).","DOI":"10.1101\/374199"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/evl3.42"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.55301.1"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202200249"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11557-010-0684-3"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2017.0392"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2011.03052.x"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12516"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069189"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru395"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5375.402"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1712337115"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","unstructured":"S. M. Latorre Museum phylogenomics of extinct Oryctes beetles from the Mascarene Islands. bioRxiv [Preprint] (2021). https:\/\/doi.org\/10.1101\/2020.02.19.954339 (Accessed 15 November 2023).","DOI":"10.1101\/2020.02.19.954339"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/bij.12553"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-019-0834-1"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa301"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01230-20"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1303\/aez.2005.53"},{"key":"e_1_3_4_42_2","first-page":"4","article-title":"Athelia arachnoidea (Berk.) J\u00fcl. and its influence on epiphytic cryptogams in urban areas","volume":"1976","author":"Arvidsson L.","year":"1975","unstructured":"L. Arvidsson, Athelia arachnoidea (Berk.) J\u00fcl. and its influence on epiphytic cryptogams in urban areas. G\u00f6teborgs Svampklubbs Arsskrift 1976, 4\u201310 (1975).","journal-title":"G\u00f6teborgs Svampklubbs Arsskrift"},{"key":"e_1_3_4_43_2","first-page":"285","article-title":"Svampangrepp p\u00e5 lavar\u2014En orsak till lav\u00f6ken","volume":"72","author":"Arvidsson L.","year":"1979","unstructured":"L. Arvidsson, Svampangrepp p\u00e5 lavar\u2014En orsak till lav\u00f6ken. Svensk Botanisk Tidskrift 72, 285\u2013292 (1979).","journal-title":"Svensk Botanisk Tidskrift"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1080\/00275514.1996.12026672"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.3767\/003158515X685283"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148228"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1922539117"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-112414-055018"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1039\/B603084J"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-011-1009-1"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2019.09.009"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2019.12.007"},{"key":"e_1_3_4_53_2","doi-asserted-by":"crossref","unstructured":"M. Meyer J. Slot The evolution and ecology of psilocybin in nature. Fungal Genet. Biol. 167 103812 (2023).","DOI":"10.1016\/j.fgb.2023.103812"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-015-1513-x"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2012.00016"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.105"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2013.12.005"},{"key":"e_1_3_4_58_2","first-page":"31","article-title":"Changes in spider webs brought about by mescaline, psilocybin and an increase in body weight","volume":"136","author":"Christiansen A.","year":"1962","unstructured":"A. Christiansen, R. Baum, P. N. Witt, Changes in spider webs brought about by mescaline, psilocybin and an increase in body weight. J. Pharmacol. Exp. Therap. 136, 31\u201337 (1962).","journal-title":"J. Pharmacol. Exp. Therap."},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00354-8"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1080\/1355621021000005937"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.funeco.2019.06.002"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2215-0366(16)30065-7"},{"key":"e_1_3_4_63_2","first-page":"47","article-title":"Thirty plus years of mushroom poisoning: Summary of the approximately 2,000 reports in the NAMA Case Registry","volume":"16","author":"Beug M.","year":"2006","unstructured":"M. Beug, M. Shaw, K. Cochran, Thirty plus years of mushroom poisoning: Summary of the approximately 2,000 reports in the NAMA Case Registry. McIlvainea 16, 47\u201368 (2006).","journal-title":"McIlvainea"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201910175"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202101382"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw354"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr947"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1183"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1400592111"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3223"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-6-31"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkf436"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1660"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx281"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-018-2129-y"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evy118"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz236"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-022-09030-5"},{"key":"e_1_3_4_84_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0197433"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg412"},{"key":"e_1_3_4_86_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-18174-1_6"},{"key":"e_1_3_4_87_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1189"},{"key":"e_1_3_4_88_2","doi-asserted-by":"publisher","DOI":"10.3732\/ajb.91.10.1446"},{"key":"e_1_3_4_89_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2009.03160.x"},{"key":"e_1_3_4_90_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw294"},{"key":"e_1_3_4_91_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl200"},{"key":"e_1_3_4_92_2","first-page":"281","article-title":"A draft reference assembly of the Psilocybe cubensis genome","volume":"10","author":"McKernan K.","year":"2021","unstructured":"K. McKernan , A draft reference assembly of the Psilocybe cubensis genome. F1000Res 10, 281 (2021).","journal-title":"F1000Res"},{"key":"e_1_3_4_93_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti079"},{"key":"e_1_3_4_94_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02100115"},{"key":"e_1_3_4_95_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026201"},{"key":"e_1_3_4_96_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2001.1862"},{"key":"e_1_3_4_97_2","doi-asserted-by":"publisher","DOI":"10.1080\/10635150290069913"},{"key":"e_1_3_4_98_2","unstructured":"W. P. Maddison D. R. Maddison Mesquite: A modular system for evolutionary analysis (2019)."},{"key":"e_1_3_4_99_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz257"},{"key":"e_1_3_4_100_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2691-7_12"},{"key":"e_1_3_4_101_2","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syy032"},{"key":"e_1_3_4_102_2","doi-asserted-by":"crossref","unstructured":"A. Bradshaw Phylogenomics of the psychoactive mushroom genus Psilocybe and evolution of the psilocybin biosynthetic gene cluster [Dataset]. Dryad. https:\/\/datadryad.org\/stash\/landing\/show?id=doi:10.5061\/dryad.tmpg4f52s. Deposited 6 November 2023.","DOI":"10.1101\/2022.12.13.520147"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2311245121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T13:47:08Z","timestamp":1704808028000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2311245121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,9]]},"references-count":102,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,1,16]]}},"alternative-id":["10.1073\/pnas.2311245121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2311245121","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.12.13.520147","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,9]]},"assertion":[{"value":"2023-07-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2311245121"}}